Population and study design
The UK Biobank is a population-based cohort of more than 500,000 participants who attended 1 of 22 assessment centers across the UK between 2006 and 2010. At enrollment, participants completed a touch-screen questionnaire, had physical measurements taken, and provided biological samples, as described in detail elsewhere [29, 30].
The current analyses were restricted to participants who had self-reported measures of walking pace and grip strength measures and without self-reported or prevalent dementia at baseline. A total of 495,700 participants were included in the final analysis (eFigure 1).
This study is based on data from the UK Biobank study that received approval from the National Information Governance Board for Health and Social Care and the National Health Service North West Multicentre Research Ethics Committee. All participants gave written informed consent before enrollment in the study, which was conducted in accord with the principles of the Declaration of Helsinki.
Exposure and covariates
The walking pace was self-reported via a touchscreen-based questionnaire by answering the question “How would you describe your usual walking pace? (i) Slow pace, (ii) Steady/average pace, and (iii) Brisk pace”. Handgrip strength was measured by using a Jamar J00105 hydraulic hand dynamometer while sitting. Isometric grip force was assessed from a single 3-s maximal grip effort of the right- and left-side arms with participants seated upright with their elbow by their side and flexed at 90° so that their forearm was facing forward and resting on an armrest. Both left and right hands strengths were measured. The mean of the right- and left-side values, expressed as kg, was used in the analysis. The measured handgrip strength was further divided into sex-specific quartiles as follows: Q1 (F: < 19.5 kg; M: < 34.0 kg), Q2 (F: 19.5 to < 23.0 kg; M: 34.0 to < 39.5 kg), Q3 (F: 23.0 to < 27.5 kg; M: 39.5 to < 45.0 kg), Q4 (F: ≥ 27.5 kg; M: ≥ 45.0 kg).
Blood collection sampling procedures for the study have previously been described and validated [30]. Biochemical assays were performed at a dedicated central laboratory. Multiple immunoassays and clinical chemistry analyzers were used to measure the biochemistry markers, details of which are provided in online companion documents (https://biobank.ndph.ox.ac.uk/ukb/ukb/docs/serum_biochemistry.pdf). Of which, C-reactive protein (CRP) levels were measured by immuno-turbidimetric method on a Beckman Coulter AU5800. Albumin levels were measured by colorimetric method on a Beckman Coulter AU5800. Body mass index (BMI) was calculated as weight (kg) by height squared (m2). Area-based socioeconomic status was derived from the postal code of residence by using the Townsend deprivation score. Smoking (never, former, current) and drinking (never or special occasions, 1–3 times/month, 1–2 times/week, 3–4 times/week, daily or almost daily) status, education levels (college or university degree, A levels/AS levels or equivalent, O levels/General Certificate of Secondary Education [GCSEs] or equivalent, Certificate of Secondary Education [CSEs] or equivalent, National Vocational Qualification [NVQ] or Higher National Diploma [NHD] or Higher National Certificates [HNC] or equivalent, other professional qualifications), and depression (yes or no) were self-reported at baseline. Family history of dementia (yes or no) was collected by answering the question “Has/did your mother/ father/ any of your brothers or sisters ever suffer from dementia?”. High education levels include college or university degrees and NVQ or NHD or HNC or equivalent. Optimal physical activity was defined as more than 4 days of vigorous/moderate physical activity in a typical week [31]. A healthy diet score [32, 33] was calculated based on the adequate intake of the following diet factors: increased consumption of fruits (≥ 3 servings/day), vegetables (≥ 3 servings/day), whole grains (≥ 3 servings/day), (shell)fish (≥ 2 servings/week), dairy products (≥ 2 servings/day), and vegetable oils (≥ 2 servings/day), and reduced or no consumption of refined grains (≤ 2 servings/day), processed meats (≤ 1 serving/week), unprocessed meats (≤ 2 servings/week), and sugar-sweetened beverages (do not drink). Each point was given for each favorable diet factor, and the healthy diet score ranged from 0 to 10. Seated blood pressure was measured twice manually (manual sphygmometer) or automatically (Omron HEM-7015IT digital blood pressure monitor), and the mean value of the two measurements was used to minimize measurement error. Hypertension was defined as systolic blood pressure (SBP) ≥ 140 mmHg, diastolic blood pressure (DBP) ≥ 90 mmHg, self-reported antihypertensive treatment or self-reported hypertension history, or International Classification of Diseases (ICD)-9 (401) or ICD-10 (I10). Prevalent diabetes (yes or no) at baseline was identified through multiple procedures considering the type of diabetes and sources of the diagnosis [34]. CVD (yes or no) at baseline was identified as a composite of preexisting coronary heart disease, myocardial infarction, ischemic heart disease, stroke, heart failure, and atrial fibrillation.
Genetic risks of dementia
The APOE genotypes were determined by a combination variant of rs429358 and rs7412. Based on the number of APOE Ɛ4 alleles, participants were divided into high-risk group (APOE ε4 dosage = 2, ε4/ε4), normal-risk group (APOE ε4 dosage = 1, ε3/ε4), and low-risk group (APOE ε4 dosage = 0, ε2/ε2, ε2/ε3, ε3/ε3) in this analysis [26, 35].
Dementia genetic risk scores (without the AOPE genotype) were calculated by 25 single nucleotide polymorphisms (SNPs), which passed quality control, based on the previous study [36]. A weighted method was used to calculate the PRS [37]; higher scores indicated a higher genetic predisposition to AD and cognitive disorder. Further detailed information on genotyping, imputation, and quality control in the UK Biobank study has been described previously [38].
Study outcomes
The primary outcome was new-onset all-cause dementia. The secondary outcomes included AD and vascular dementia. Diagnoses were recorded using the International Classification of Diseases (ICD9 and ICD10) coding system (eTable 1). The accuracy of dementia ascertainment has been validated previously [39].
Each participant’s person-years were calculated from the date of attending the assessment center to the date reported for diagnosis of new-onset dementia events, death, loss to follow-up, or end of the follow-up, whichever occurred first.
Statistical analysis
Baseline characteristics were presented as mean (SD) for continuous variables or proportions for categorical variables by walking pace and sex-specific quartiles of handgrip strength. Differences in characteristics were compared using ANOVA tests or chi-square tests accordingly.
Basic demographic variables and variables known as traditional or suspected risk factors for dementia were selected as covariates. The relations of walking pace, handgrip strength with all-cause dementia, and the major subtypes of dementia (AD and vascular dementia) were estimated using Cox proportional hazards models (hazards ratio [HR] and 95% confidence interval [CI]) without (the crude model) and with adjustments for a series of covariates, including age, sex, ethnicities, Townsend deprivation score, BMI, smoking and alcohol drinking status, education levels, physical activity, healthy diet scores, CRP, albumin, CVD, hypertension, depression, diabetes, and family history of dementia in model 1. Moreover, APOE ε4 dosage and dementia genetic risk scores were further adjusted in model 2 and model 3, respectively. In model 4, walking pace and handgrip strength were further mutually adjusted.
We combined walking pace and handgrip strength into a category variable: group 1, slow walking pace and grip strength in the first quartile; group 2, slow walking pace and grip strength in the 2–4 quartiles; group 3, average or brisk walking pace and grip strength in the first quartile; group 4, average or brisk walking pace and grip strength in the 2–4 quartiles. Similar Cox proportional hazards models were used to examine the association of combined walking speed and handgrip strength with risk of all-cause dementia with group 1 as reference.
To evaluate the interactions between walking pace and age, APOE ε4 dosage, handgrip strength, or other confounding factors (BMI, education, physical activity, hypertension, depression, diabetes, and family history of dementia), multiplicative interactions were assessed by adding an interaction term to the Cox proportional hazards model.
A two-tailed P < 0.05 was considered to be statistically significant in all analyses. R software (version 4.1.3, http://www.R-project.org) was used for all statistical analyses.