Re-examining tau-immunoreactive pathology in the population: granulovacuolar degeneration and neurofibrillary tangles
© Hunter et al. 2015
Received: 16 December 2014
Accepted: 11 August 2015
Published: 28 August 2015
Alzheimer’s disease (AD) is associated with neurofibrillary pathology, including neurofibrillary tangles (NFT), neuritic plaques (NP) and neuropil threads containing aggregated microtubule associated protein tau. Aggregated tau is also associated with granulovacuolar degeneration (GVD). The relationships between tau, GVD, NFT and dementia are unclear.
We assessed hippocampal (CA1) tau-immunoreactive GVD and NFT pathology in brain donations from the population-representative Cambridge City over 75s Cohort (CC75C) using the CERAD protocol and a modified protocol that included a morphological characterisation of tau-immunoreactive deposits within neurons as NFTs or as GVD. Associations between GVD, NFT and dementia were investigated.
Hippocampal pyramidal neurons affected with either NFT or GVD are common in the older population. Some tau-immunoreactive deposits resemble ghost GVD neurons. Tau immunoreactivity identified GVD in 95 % cases rated as none with haematoxylin and eosin staining. Both severe NFT (odds ratio (OR) 7.33, 95 % confidence interval (CI) 2.01; 26.80, p = 0.003) and severe GVD (OR 7.48, 95 %(CI) 1.54; 36.24, p = 0.012) were associated with dementia status. Increasing NFT (OR 2.47 95 %(CI) 1.45; 4.22, p = 0.001) and GVD (OR 2.12 95 %(CI) 1.23; 3.64, p = 0.007) severities are associated with increasing dementia severity. However, when the analyses were controlled for other neuropathologies (NFT, NP, Tar-DNA binding Protein-43 and amyloid deposits), the associations between GVD and dementia lost significance.
Current neuropathological assessments do not adequately evaluate the presence and severity of the GVD pathology and its contribution to dementia remains unclear. We recommend that protocols to assess GVD should be developed for routine use and that tau, in a non-PHF associated conformation, is reliably associated with GVD.
Alzheimer’s disease (AD) is characterised clinically by memory loss, cognitive impairment and behavioural problems , and neuropathologically by neuronal and synaptic loss and by various deposits containing the amyloid-beta protein (Aβ) and aggregated microtubule-associated protein tau in the form of neurofibrillary pathologies including neurofibrillary tangles (NFT), dystrophic neurites in neuritic plaques (NP) and neuropil threads (NT) . Assessments of these lesions form the basis for the neuropathological confirmation of a clinical diagnosis of probable AD according to the Consortium to Establish a Registry for Alzheimer’s Disease (CERAD) protocol [2, 3] and the assessment of tau-immunoreactive (IR) changes according to Braak stage [4, 5]. The assessments for neuropathologically diagnosing AD have been updated recently to include assessments of Aβ deposits [6, 7].
The tau-associated pathologies, visualised by various methods and assessed using both the CERAD protocol [2, 3] and Braak staging [5, 8], include NFT, NP and NT. NFT are intracellular fibrous inclusions composed of paired helical filaments (PHFs) of aggregated and often hyperphosphorylated tau . The CERAD protocol [2, 3] counts only those neurons with mature, robustly staining NFT; those neurons with diffuse or granular staining are classified as pre-tangles and are not counted. The morphology of tau-IR cytoplasmic inclusions is not considered. The presence of extracellular ghost NFT, the remains of NFT after the neuron has died, are noted in both CERAD and Braak staging. These are assessed on haematoxylin and eosin (H & E) or silver stained slides because many tau-IR epitopes associated with NFT are lost .
In addition to the well-recognised AD-associated pathologies and argyrophilic grains in argyrophilic grain disease (AGD) , various studies have demonstrated that the dense granules of granulovacuolar degeneration (GVD) also react with tau antibodies directed against various epitopes [10, 12–15] but not those directed against some conformational epitopes specific for PHFs [16, 17]. The possibility of potential confusion relating to the interpretation of tau-IR pathology has been noted previously . GVD, first described by Teofil Simchowicz , is characterised by neurons with two or more double membrane-bound cytoplasmic vacuoles containing an electron-dense granule  and are usually assessed on H & E slides. GVD is noted in the CERAD protocol as present or absent in the hippocampus [2, 3]. GVD is distinct from other tau-IR granules described previously that lack membrane enclosed vacuoles and have been interpreted as PHF core bodies .
GVD within hippocampal pyramidal cells has previously been associated with increasing age and AD [21–23] and is associated with intraneuronal accumulation of tau protein . GVD is also found in other neurodegenerative disorders, including Pick’s disease, multiple system atrophy with Parkinsonism, amyotrophic lateral sclerosis with dementia, Down syndrome and progressive supranuclear palsy [15, 24, 25]. Previous work has suggested a possible progression of development for GVD [23, 26], ranging from a few cytoplasmic granulovacuolar inclusions to neurons completely filled with granulovacuolar inclusions, which may follow topographical stages [15, 27]. While the association of GVD with AD has long been noted [12, 21], a recent study estimating the contributions of rare or disregarded pathologies  found no significant association between GVD and dementia when plaques and tangles were controlled for—raising questions relating to the significance of GVD to dementia.
The literature reviewed suggests that the relationship between GVD and dementia is unclear and that there is potential for confusion in interpretations of tau-IR pathologies. We examined the pathology of tau-IR GVD and NFT in detail in the cornus ammonis (CA) fields of the hippocampus in the population-based Cambridge City over-75s Cohort (CC75C) with an antibody against the C-terminal portion of tau that is not dependent on phosphorylation or PHF-specific epitopes. We categorised neuronal tau pathology as NFT, GVD or mixed (NFT+GVD), and investigated how this detailed morphology relates to current assessments of GVD and tau pathology according to well-accepted protocols and relates to the associations between NFT, GVD and dementia.
Materials and methods
The study sample
CC75C is a population-based, longitudinal study of aging and dementia with a brain donation programme . Each study participant gave consent to participate and for brain donation. Consent for brain donation was also given by next of kin. Each phase of the CC75C study has been approved by the Cambridge Research Ethics Committee.
At the time of this study a total of 237 brain donations had CERAD measures, of which 211 cases were included for all analyses. Twenty five slides were not available or the area of interest was not present on the slide to score tau-IR GVD, NFT and GVD+NFT. For one slide, CA1 was not evaluable owing to complete neuronal loss.
A consensus diagnosis for dementia status at death consistent with Diagnostic and Statistical Manual of Mental Disorders 4th edition (DSM-IV) criteria  was agreed by clinicians, blinded to neuropathology reports, using post-mortem review of all clinical information including proxy informant data, death certificates and retrospective informant data after death. Dementia severity was rated as none, minimal, mild, moderate and severe . Where dementia severity could not be rated it was scored as unknown.
Brain collection for this study occurred between 1989 and 2009. After death, the brains were removed as soon as possible in the local mortuary. The brains were bisected in the sagittal plane. One cerebral hemisphere was dissected coronally into approximately 1 cm slices, macroscopically examined and then snap-frozen to –80 °C. The other half of the brain was fixed in formalin for at least 6 weeks and dissected coronally into approximately 1 cm slices. For diagnostic purposes, tissue blocks for paraffin embedding were taken from the hippocampus (at the level of the lateral geniculate body), entorhinal cortex (at the level of the mammillary body), frontal, temporal, parietal and occipital lobes, basal ganglia, thalamus, pons, medulla, cerebellum and two levels of the midbrain. Serial sections from the paraffin-embedded brain tissue samples were assessed for neuropathology blind to clinical status according to the CERAD protocol [2, 3] and Braak stage [4, 5]. All slides were produced by the Cambridge Brain Bank, Cambridge, UK and assessments were performed blind to clinical status by neuropathologists at Addenbrooke’s Hospital, Cambridge, UK. All immunostained sections for diagnosis were counterstained with Ehrlich’s haematoxylin with diaminobenzidine as the chromagen.
Lewy bodies were assessed as present or absent using 10 μm sections stained with H & E in combination with slides stained with anti-ubiquitin antibody (pAb BR 251, Z0458; DAKO, Glostrup, Denmark) (first 174 donations) or with anti-α-synuclein (SA3400; Biomol International, Enzo Life Sciences, Farmingdale, New York, USA) (last 63 cases). Aβ deposits as senile plaques and cerebral amyloid angiopathy (CAA) were visualised with Congo red and/or anti-Aβ antibody (M872, Clone 6 F/3D; DAKO) on 10 μm sections and assessed as none, mild, moderate or severe according to the CERAD protocol .
Inclusions reactive with tar-DNA binding protein 43 (TDP-43) were assessed using 9 μm sections from the hippocampus and entorhinal cortex stained with anti-TDP-43 antibody (pS409/410-2; Cosmo Bio Co. Ltd, Tokyo, Japan). Slides were counterstained with Harris’ haematoxylin with diaminobenzidine as the chromagen. Solid neuronal inclusions were assessed, based on the protocol in Neumann et al. , as none (no inclusions), minimal (one inclusion per slide), mild (one or more inclusions in up to half the fields of view per slide), moderate (a few inclusions in over half the fields of view per slide) or severe (a few inclusions in most fields of view per slide).
Sections 10 μm thick from the hippocampus and entorhinal cortex were immunostained with anti-tau monoclonal antibody (mAb) 11.57 (supplied by The Cambridge Brain Bank, dilution 1:5; gift donated by Professor Claude Wischik, University of Aberdeen, UK) to visualise NFT, NP and dystrophic neurites. mAb 11.57 was raised against a pronase-treated PHF core sub-fraction and recognises a phosphorylation and conformation-independent epitope in the C-terminal region of tau . mAb 11.57 recognises NFT, NP, NT and GVD bodies and the grains of AGD but not ghost NFT [12, 33]. During the diagnostic process, an experienced neuropathologist scored the severity of NFT according to the CERAD protocol [2, 3] as either none, mild, moderate or severe. GVD, defined as neurons with two or more membrane-bound cytoplasmic vacuoles containing a basophilic granule, was rated according to the CERAD protocol [2, 3] as present or absent on hippocampal sections 10 μm thick stained with H & E.
A second protocol was designed to assess the separate tau-IR pathologies based on morphology. Tau-IR NFT (defined as neurons with fibrous deposits of tau and no vacuoles containing an electron dense granule), tau-IR GVD (defined as neurons with two or more membrane-bound cytoplasmic vacuoles containing a tau-IR dense granule and no fibres) and neurons containing mixed pathology with both fibrous and granulovacuolar pathology (NFT+GVD) were scored at 100× magnification as none, an isolated example per area, and as mild, moderate or severe by comparison with the references images for NFT from the CERAD protocol . The same slides were assessed with both protocols. Higher magnifications (200× and 400×) were used to assess the detailed morphology of any tau-IR structures that could not be classified at lower magnification (100×). Separate scores for the hippocampal regions CA1 and CA2–3, the regions most associated with tau immunoreactivity, were generated by SH and inter-rated by TP. Regions CA2 and CA3 were combined for scoring owing to difficulties in reliably defining the CA2–CA3 boundary. Photomicrographs were taken with a Leica DM LB microscope with a DC500 digital camera Wetzlar, Germany.
Inter-rater reliability on tau-IR GVD, NFT and GVD+NFT by hippocampal area
95 % CI (AC2)
−0.33 to 0.58
Inter-rater reliability was not substantial for NFT+GVD in CA2–3, so further analyses were restricted to NFT and GVD in the CA1 area only. The mean (standard deviation) is presented when applicable.
The relationships between tau-IR GVD and NFT with dementia status were verified using logistic regression. Dementia status was defined as the dependent variable and tau-IR scores in CA1 as independent variables. Three hierarchical models were created: model 1 controlled for sex and age at death; model 2 was additionally controlled for NFT and NP; and model 3 additionally controlled for TDP-43 and amyloid deposits. The same approach was used to verify the relationships between tau-IR GVD and NFT with dementia severity, using ordinal logistic regression.
The Spearman coefficient (r) was calculated to verify the relationships between different pathologies and different protocols. All tests were two-tailed. Statistical analyses were performed using statistical package STATA, version 12 College Station, Texas, USA.
In total, 211 brains were included in the sample. Among them, 147 (70 %) were women. Mean age of death was 90.97 (4.51) years. Dementia status at death could not be determined in eight (4 %) participants, 71 (34 %) did not have dementia and 132 (63 %) had dementia at death.
Both NFT and GVD were common; using the new protocol, 91 % of the cases surveyed showed both GVD and NFT in the hippocampal CA1 fields, 1 % had NFT with no GVD, 4 % had GVD with no NFT and 4 % had neither NFT nor GVD pathology.
Relationship between CERAD assessments and the new protocol
Comparison of GVD scores from the two protocols
CERAD protocol GVD score
Tau-IR GVD scorea
Comparison of NFT scores from the two protocols
CERAD protocol NFT score
Tau-IR NFT scorea
Relationship between NFT and GVD
Relationship between CERAD scores for NFT and GVD
CERAD protocol NFT score
CERAD protocol GVD score
There was no significant correlation between NFT and GVD assessed with the CERAD protocol (n = 126, r = −0.03, p = 0.760).
Relationship between new protocol scores for NFT and GVD
NFT score from tau-IR protocol
GVD score from tau-IR protocol
There was moderate correlation between NFT and GVD using the new tau-IR protocol (n = 211, r = 0.65, p <0.001).
Relationships between dementia and tau-IR pathologies
Analyses for tau-IR were conducted on 211 brain donations. Only two cases with hippocampal Lewy body pathology and only a few cases with moderate/severe CAA were identified; these neuropathologies were therefore not included in further analyses. The distributions between dementia severity and various hippocampal neuropathologies are shown in Additional file 1.
Logistic regression models showing the relationships between hippocampal pathologies and presence of clinical dementia
Ordinal logistic regression models showing the relationships between hippocampal pathologies and severity of clinical dementia
Descriptions of tau-IR pathology
Very rarely, neurons with GVD also contained one or more discrete fibrous bundles (Fig. 1b, neuron 5) that could not be classified as NFT or GVD/NFT mixed and so were not included in the assessments. Pyramidal neurons with diffuse cytoplasmic tau reactivity, often with a defined line around the nucleus (Fig. 1b, neurons 4) and previously described in association with AGD , seen in the hippocampal areas CA1, CA2 and CA3 were also not included in assessments for this study.
We found more GVD pathology using tau-IR than was detected by H & E, suggesting that GVD is missed with current protocols. The associations between dementia and both hippocampal (CA1) NFT and GVD are significant. Additionally, the severity of both NFT and GVD were significantly associated with dementia severity. However, the association between GVD and dementia lost significance when NFT, NP and other pathologies were controlled for.
We find that there is a potential for misinterpretation of tau pathology. Since many anti-tau antibodies react with both NFT and GVD, there is potential for neurons with GVD to be mistaken for pre-tangles and neurons with severe GVD to be mistaken for NFT at low magnifications. We also found tau-IR deposits resembling ghost GVD neurons that appeared morphologically different from ghost NFT.
This study has limitations. The selection of anti-tau immunostained slides used in this study was from the historical diagnostic collection and assessments were semi-quantitative and not stereological ; this may introduce bias because we assume a single slide will represent a specific case adequately. Since comparison of the scoring protocols required that we use the same diagnostic slides, missing slides—perhaps due to breakage over the ~25 years of the study to date—may introduce bias.
Our assessments of Lewy bodies are based on older protocols for 174/241 cases and therefore scores for Lewy bodies might be underestimates. Both hippocampal Lewy bodies and CAA were rare and not included in analyses.
In any study of this type, brains donated at death represent a cross-section of the cohort at a specific terminal point. While the presentation of GVD morphology in Fig. 2 appears to represent a progression of increasing severity, this is not certain. Additionally, the morphological similarity of structures in Fig. 3 to ghost GVD neurons is circumstantial. These interpretations would need to be confirmed by longitudinal studies.
Of the 211 cases included in our study, only 126 had measures for GVD from the CERAD protocol. It is possible that GVD was not seen on H & E staining and therefore no measure was recorded. Alternatively, historically GVD has not been prioritised as a dementia-related pathology and therefore GVD assessments may have been neglected. We did not use a second marker for GVD; however, we were very careful to count only neurons containing two or more structures with a dense granule surrounded by the halo of a vacuole and it is unlikely that we have overestimated GVD. A marker for GVD has yet to be validated and, given our poor correlations between GVD and NFT as assessed using the CERAD protocol, a consensus regarding the reliability of GVD markers is required.
Tau-IR reliably marks nearly all dense granules associated with GVD and we identified more cases with tau-IR GVD than with the standard assessment protocol using H & E staining. Similarly, a study using antibodies to tubulin found four times more GVD than H & E alone , and the phosphorylated ribosomal protein S6 (pS6) revealed more neurons containing both NFT and GVD than in our study , suggesting that GVD can be missed in standard neuropathological protocols using H & E. Thal et al.  suggest that tau is not a good marker for GVD owing to its involvement with various pathologies, and another candidate marker for GVD (pS6) revealed more neurons containing both NFT and GVD than in our study ; therefore it is possible that GVD-IR is masked by immunoreactivity for NFT and that our measures underestimate GVD when present in NFT.
Our more detailed assessment of tau-IR NFT correlated with that of the CERAD protocol moderately. The CERAD score represents the highest score measured over the entire hippocampal structure and our detailed score is for CA1 only. However, since CA1 is often the most severely affected hippocampal structure [4, 5], we would expect good correlation. If the morphology of tau-IR is not considered, there is potential for occasional neurons severely affected by GVD and neurons with both GVD and NFT to be misclassified as pure NFT, as illustrated in Figs. 1 and 2. This may also contribute to the moderate correlation found.
We found a significant association between both NFT and GVD with dementia. However, when NFT and NP were controlled for, the association between GVD and dementia lost significance. This is in agreement with results from the study by Keage et al. . In agreement with Ball , our results suggest that GVD severity increases as NFT severity increases. A loss of significance can mean that GVD is not an independent contributor to dementia and so is ‘unimportant’ or that there is some mechanism involving GVD, NFT and plaques that undermines the assumption of independence for this statistical test. With increasing evidence that NFT and GVD share some features, including tau, and not others (e.g., TDP-43 ), it is possible that loss of significance reflects some shared process(es) rather than GVD not being an important contributor to dementia. At a mechanistic level, various studies imply that GVD is a pre-tangle and/or contributes to early NFT formation [42, 43]. This conflicts with other studies , including ours, which present evidence that GVD can be interpreted as a separate process.
Our estimates of the associations between both severities of GVD and NFT and dementia status may be unreliable because only CA1 scores were included. For a future detailed investigation of the relationships between NFT, GVD and dementia, neocortical and subcortical areas should be included .
In agreement with Bondareff et al. , in each available case from the CC75C cohort affected by both NFT and GVD we have observed neurons with classic fibrillar NFT only, classic GVD only and, more rarely, neurons with both NFT and GVD (Figs. 1 and 2). While these pathologies can rarely occur together in the same neuron, we do not see neurons in which GVD-like deposits can be interpreted as developing into NFT following the generally accepted progression from pre-tangle to mature NFT to ghost tangle as the CERAD protocol and other studies imply [42, 43]. Rather, NFT and GVD appear to have their own progression in severity (Fig. 2) and their own ghost forms (Figs. 2 and 3). Given the associations between GVD and NFT and dementia presented, our evidence supports previous suggestions that the formation of NFT and GVD are separate processes that can co-occur in any individual .
In comparison, the accepted longitudinal progression for NFT from pre-tangles to mature tangles to extracellular ghosts is also based on similar cross-sectional studies. Our findings suggest that the widely accepted progression of NFT may not be adequate to explain the variations in tau-IR we present. We suggest that the pathways of both granulovacuolar and neurofibrillary tau-IR pathology require careful evaluation to separate and understand the relationships between these two processes and their involvement in dementia. Additional processes may relate to diffuse tau-IR seen in neurons marked in Fig. 2 and these may be associated with AGD . Definitions of what a pre-tangle is need clarification.
Most GVD granules are labelled strongly with tau, suggesting that tau is involved in the development of GVD at an early stage. GVD shows an amorphous structure on electron micrographs [14, 19], shows no fluorescence with thioflavin S  and is not marked by mAbs such as 7.51 , Alz-50 [25, 44], Tau-1  and PHF-1 [16, 45] recognising epitopes specific to PHFs. The above evidence suggests that PHFs, typical of NFT, are not contained in GVD. Tau aggregation in GVD has been interpreted as representing a second pathway of tau aggregation . Tau as a component of GVD will be missed when using PHF reactive antibodies. Why some neurons develop NFT, GVD or occasionally both is not known but could arise owing to different stress responses in different neuronal subtypes, different neurodegenerative processes or different stress responses within the neurons. Investigations into the initiation and development of GVD may offer alternative therapeutic targets.
Tau in a non-PHF-associated conformation is reliably associated with GVD. Current neuropathological protocols do not adequately capture the extent of tau-IR pathology and require re-evaluation, in particular, to better assess the contribution of GVD to dementia and differentiate it from pathology of NFT. The relationships between NFT, GVD and pre-tangles are unclear, which has consequences for investigations into tau aggregation, especially at early stages of disease. We recommend that protocols to assess GVD should be developed for routine use.
Argyrophilic grain disease
Cerebral amyloid angiopathy
Cambridge City over 75s Cohort
Consortium to Establish a Registry for Alzheimer’s Disease
Diagnostic and Statistical Manual of Mental Disorders 4th edition
- H & E:
Haematoxylin and eosin
Paired helical filament
Phosphorylated ribosomal protein S6
Tar-DNA binding protein 43
The authors thank all of the participants, families and carers in the CC75C study. CC75C is a member study of the Cambridgeshire and Peterborough Collaboration for Leadership in Applied Health Research and Care (CLAHRC). The Cambridge Brain Bank Laboratory (which processed all CC75C cases) is supported by the National Institute for Health Research, Cambridge BioMedical Research Centre. EM-L is supported by the Alzheimer’s Society (London, UK).
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Morris JC, Heyman A, Mohs RC, Hughes JP, van Belle G, Fillenbaum G, et al. The Consortium to Establish a Registry for Alzheimer's Disease (CERAD). Part I. Clinical and neuropsychological assessment of Alzheimer's disease. Neurology. 1989;39:1159–65.View ArticlePubMedGoogle Scholar
- Mirra SS, Heyman A, McKeel D, Sumi SM, Crain BJ, Brownlee LM, et al. The Consortium to Establish a Registry for Alzheimer's Disease (CERAD). Part II. Standardization of the neuropathologic assessment of Alzheimer's disease. Neurology. 1991;41:479–86.View ArticlePubMedGoogle Scholar
- Consortium to Establish a Registry for Alzheimer Disease Neuropathological Assessment. http://cerad.mc.duke.edu/Neuropathology.htm. Accessed 24/08/2015.
- Braak H, Braak E. Neuropathological stageing of Alzheimer-related changes. Acta Neuropathol. 1991;82:239–59.View ArticlePubMedGoogle Scholar
- Braak H, Alafuzoff I, Arzberger T, Kretzschmar H, Del Tredici K. Staging of Alzheimer disease-associated neurofibrillary pathology using paraffin sections and immunocytochemistry. Acta Neuropathol. 2006;112:389–404.View ArticlePubMed CentralPubMedGoogle Scholar
- Hyman BT, Phelps CH, Beach TG, Bigio EH, Cairns NJ, Carrillo MC, et al. National Institute on Aging-Alzheimer's Association guidelines for the neuropathologic assessment of Alzheimer's disease. Alzheimers Dement. 2012;8:1–13.View ArticlePubMed CentralPubMedGoogle Scholar
- Montine TJ, Phelps CH, Beach TG, Bigio EH, Cairns NJ, Dickson DW, et al. National Institute on Aging-Alzheimer's Association guidelines for the neuropathologic assessment of Alzheimer's disease: a practical approach. Acta Neuropathol. 2012;123:1–11.View ArticlePubMed CentralPubMedGoogle Scholar
- Braak H, Braak E. Staging of Alzheimer's disease-related neurofibrillary changes. Neurobiol Aging. 1995;16:271–8. discussion 278–84.View ArticlePubMedGoogle Scholar
- Harrington CR, Mukaetova-Ladinska EB, Hills R, Edwards PC, Montejo De Garcini E, Novak M, et al. Measurement of distinct immunochemical presentations of tau protein in Alzheimer disease. Proc Natl Acad Sci U S A. 1991;88:5842–6.View ArticlePubMed CentralPubMedGoogle Scholar
- Bondareff W, Wischik CM, Novak M, Amos WB, Klug A, Roth M. Molecular analysis of neurofibrillary degeneration in Alzheimer's disease. An immunohistochemical study. Am J Pathol. 1990;137:711–23.PubMed CentralPubMedGoogle Scholar
- Braak H, Braak E. Argyrophilic grain disease: frequency of occurrence in different age categories and neuropathological diagnostic criteria. J Neural Transm. 1998;105:801–19.View ArticlePubMedGoogle Scholar
- Bondareff W, Wischik CM, Novak M, Roth M. Sequestration of tau by granulovacuolar degeneration in Alzheimer's disease. Am J Pathol. 1991;139:641–7.PubMed CentralPubMedGoogle Scholar
- Dickson DW, Ksiezak-Reding H, Davies P, Yen SH. A monoclonal antibody that recognizes a phosphorylated epitope in Alzheimer neurofibrillary tangles, neurofilaments and tau proteins immunostains granulovacuolar degeneration. Acta Neuropathol. 1987;73:254–8.View ArticlePubMedGoogle Scholar
- Ikegami K, Kimura T, Katsuragi S, Ono T, Yamamoto H, Miyamoto E, et al. Immunohistochemical examination of phosphorylated tau in granulovacuolar degeneration granules. Psychiatry Clin Neurosci. 1996;50:137–40.View ArticlePubMedGoogle Scholar
- Thal DR, Del Tredici K, Ludolph AC, Hoozemans JJ, Rozemuller AJ, Braak H, et al. Stages of granulovacuolar degeneration: their relation to Alzheimer's disease and chronic stress response. Acta Neuropathol. 2011;122:577–89.View ArticlePubMedGoogle Scholar
- Holzer M, Gartner U, Stobe A, Hartig W, Gruschka H, Bruckner MK, et al. Inverse association of Pin1 and tau accumulation in Alzheimer's disease hippocampus. Acta Neuropathol. 2002;104:471–81.PubMedGoogle Scholar
- Love S, Saitoh T, Quijada S, Cole GM, Terry RD. Alz-50, ubiquitin and tau immunoreactivity of neurofibrillary tangles, Pick bodies and Lewy bodies. J Neuropathol Exp Neurol. 1988;47:393–405.View ArticlePubMedGoogle Scholar
- Simchowicz T. Histologische Studien uber die senile Demenz. Histologische und histopathologische Arbeiten uber die Grosshirnrinde (Histological studies of senile dementia. Histological and histopathological work over the cerebral cortex.). 1911;4:267–444.
- Okamoto K, Hirai S, Iizuka T, Yanagisawa T, Watanabe M. Reexamination of granulovacuolar degeneration. Acta Neuropathol. 1991;82:340–5.View ArticlePubMedGoogle Scholar
- Mena R, Edwards PC, Harrington CR, Mukaetova-Ladinska EB, Wischik CM. Staging the pathological assembly of truncated tau protein into paired helical filaments in Alzheimer's disease. Acta Neuropathol. 1996;91:633–41.View ArticlePubMedGoogle Scholar
- Ball MJ, Lo P. Granulovacuolar degeneration in the ageing brain and in dementia. J Neuropathol Exp Neurol. 1977;36:474–87.View ArticlePubMedGoogle Scholar
- Xu M, Shibayama H, Kobayashi H, Yamada K, Ishihara R, Zhao P, et al. Granulovacuolar degeneration in the hippocampal cortex of aging and demented patients—a quantitative study. Acta Neuropathol. 1992;85:1–9.View ArticlePubMedGoogle Scholar
- Tomlinson BE, Kitchener D. Granulovacuolar degeneration of hippocampal pyramidal cells. J Pathol. 1972;106:165–85.View ArticlePubMedGoogle Scholar
- Yamazaki Y, Matsubara T, Takahashi T, Kurashige T, Dohi E, Hiji M, et al. Granulovacuolar degenerations appear in relation to hippocampal phosphorylated tau accumulation in various neurodegenerative disorders. PLoS ONE. 2011;6:e26996.View ArticlePubMed CentralPubMedGoogle Scholar
- Schwab C, DeMaggio AJ, Ghoshal N, Binder LI, Kuret J, McGeer PL. Casein kinase 1 delta is associated with pathological accumulation of tau in several neurodegenerative diseases. Neurobiol Aging. 2000;21:503–10.View ArticlePubMedGoogle Scholar
- Mann DM. Granulovacuolar degeneration in pyramidal cells of the hippocampus. Acta Neuropathol. 1978;42:149–51.View ArticlePubMedGoogle Scholar
- Ball MJ. Topographic distribution of neurofibrillary tangles and granulovacuolar degeneration in hippocampal cortex of aging and demented patients. A quantitative study. Acta Neuropathol. 1978;42:73–80.View ArticlePubMedGoogle Scholar
- Keage HA, Ince PG, Matthews FE, Wharton SB, McKeith IG, Brayne C, et al. Cc75C: Impact of less common and ‘disregarded’ neurodegenerative pathologies on dementia burden in a population-based cohort. J Alzheimers Dis. 2012;28:485–93.PubMedGoogle Scholar
- Fleming J, Zhao E, O'Connor DW, Pollitt PA, Brayne C. Cohort profile: the Cambridge City over-75s Cohort (CC75C). Int J Epidemiol. 2007;36:40–6.View ArticlePubMedGoogle Scholar
- APA. Diagnostic and statistical manual of mental disorders. DSM-III-R. Washington: American Psychiatric Association; 1987.Google Scholar
- Roth M, Tym E, Mountjoy CQ, Huppert FA, Hendrie H, Verma S, et al. CAMDEX. A standardised instrument for the diagnosis of mental disorder in the elderly with special reference to the early detection of dementia. Br J Psychiatry. 1986;149:698–709.View ArticlePubMedGoogle Scholar
- Neumann M, Sampathu DM, Kwong LK, Truax AC, Micsenyi MC, Chou TT, et al. Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Science. 2006;314:130–3.View ArticlePubMedGoogle Scholar
- Hodges JR, Davies RR, Xuereb JH, Casey B, Broe M, Bak TH, et al. Clinicopathological correlates in frontotemporal dementia. Ann Neurol. 2004;56:399–406.View ArticlePubMedGoogle Scholar
- Gwet KL. Computing inter-rater reliability and its variance in the presence of high agreement. Br J Math Stat Psychol. 2008;61:29–48.View ArticlePubMedGoogle Scholar
- Landis JR, Koch GG. The measurement of observer agreement for categorical data. Biometrics. 1977;33:159–74.View ArticlePubMedGoogle Scholar
- Kovacs GG, Budka H. Current concepts of neuropathological diagnostics in practice: neurodegenerative diseases. Clin Neuropathol. 2010;29:271–88.View ArticlePubMedGoogle Scholar
- Bussiere T, Gold G, Kovari E, Giannakopoulos P, Bouras C, Perl DP, et al. Stereologic analysis of neurofibrillary tangle formation in prefrontal cortex area 9 in aging and Alzheimer's disease. Neuroscience. 2003;117:577–92.View ArticlePubMedGoogle Scholar
- Price DL, Altschuler RJ, Struble RG, Casanova MF, Cork LC, Murphy DB. Sequestration of tubulin in neurons in Alzheimer's disease. Brain Res. 1986;385:305–10.View ArticlePubMedGoogle Scholar
- Castellani RJ, Gupta Y, Sheng B, Siedlak SL, Harris PL, Coller JM, et al. A novel origin for granulovacuolar degeneration in aging and Alzheimer's disease: parallels to stress granules. Lab Invest. 2011;91:1777–86.View ArticlePubMed CentralPubMedGoogle Scholar
- Ball MJ. Neuronal loss, neurofibrillary tangles and granulovacuolar degeneration in the hippocampus with ageing and dementia. A quantitative study. Acta Neuropathol. 1977;37:111–8.View ArticlePubMedGoogle Scholar
- Kadokura A, Yamazaki T, Kakuda S, Makioka K, Lemere CA, Fujita Y, et al. Phosphorylation-dependent TDP-43 antibody detects intraneuronal dot-like structures showing morphological characters of granulovacuolar degeneration. Neurosci Lett. 2009;463:87–92.View ArticlePubMedGoogle Scholar
- Kohler C, Dinekov M, Gotz J. Granulovacuolar degeneration and unfolded protein response in mouse models of tauopathy and Abeta amyloidosis. Neurobiol Dis. 2014;71:169–79.View ArticlePubMedGoogle Scholar
- Lund H, Gustafsson E, Svensson A, Nilsson M, Berg M, Sunnemark D, et al. MARK4 and MARK3 associate with early tau phosphorylation in Alzheimer's disease granulovacuolar degeneration bodies. Acta Neuropathol Commun. 2014;2:22.View ArticlePubMed CentralPubMedGoogle Scholar
- Carmel G, Mager EM, Binder LI, Kuret J. The structural basis of monoclonal antibody Alz50's selectivity for Alzheimer's disease pathology. J Biol Chem. 1996;271:32789–95.View ArticlePubMedGoogle Scholar
- Yamaguchi H, Nakazato Y, Kawarabayashi T, Ishiguro K, Ihara Y, Morimatsu M, et al. Extracellular neurofibrillary tangles associated with degenerating neurites and neuropil threads in Alzheimer-type dementia. Acta Neuropathol. 1991;81:603–9.View ArticlePubMedGoogle Scholar