Fig. 1

Reduction of EphA4 protein and mRNA levels in the hippocampus and cortex of APPPS1 mice. Representative images (a) and quantification (b) of a Western blot analysis of hippocampal EphA4 protein levels in Ctrl and EphA4-KO mice (upper blot) and AD and AD;EphA4-KO mice (lower blot) with GAPDH protein levels as a loading control (unpaired t test and Mann-Whitney test respectively, n = 4–8 mice/group). Representative images (c) and quantification (d) of a Western blot analysis of cortical EphA4 protein levels in Ctrl and EphA4-KO mice (upper blot) and AD and AD;EphA4-KO mice (lower blot). GAPDH protein levels were assessed to control for equal loading (unpaired t test, n = 4 mice/group). e Representative images of RNA scope with specific probes for EphA4 in the DG, CA3, and CA1 regions of the hippocampus of Ctrl and EphA4-KO mice. Hoechst was used to stain cell nuclei. *p ≤ 0.05, **p ≤ 0.01. Scale bar = 100 μm. Abbreviation: Ctrl control