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Selectivity to amyloid-β precursor protein cleavage provides hope against Alzheimer's
Alzheimer's Research & Therapy volume 3, Article number: 7 (2011)
Toward development of a safe and effective treatment for Alzheimer's disease, Elan Pharmaceuticals reported a novel γ-secretase inhibitor that specifically targets the cleavage of amyloid-β precursor protein, opening the way to design of substrate-specific γ-secretase inhibitors that would reduce the amyloid burden without significant adverse events.
As the population in most industrialized nations ages, the development of disease-modifying agents against Alzheimer's disease (AD), the most common cause of dementia in the elderly, is one of the most important unmet medical needs in the world. Because of strong experimental support for amyloid-β (Aβ), a small peptide derived from the sequential cleavage of amyloid-β pre-cursor protein (APP) by BACE1 and γ-secretase, as the principle culprit in this devastating neurodegenerative disease, investigators focused efforts to develop com-pounds designed to inhibit the activity of γ-secretase in order to attenuate β-amyloidosis as a potential therapy for AD . However, Eli Lilly and Company announced last year that it was halting the development of semagacestat, a first-in-man γ-secretase inhibitor [2, 3]. Two ongoing phase III studies showed that treatments using semagacestat were associated with worsening of clinical measures of cognition and the ability to perform activities of daily living, as well as an increased risk of skin cancer. The exact molecular mechanisms underlying these adverse events remain unclear. However, semagacestat mechanistically inhibits not only γ-secretase to prevent Aβ production but also Notch, one of the most important physiological substrates for γ-secretase in vivo ; genetic and pharmacological studies in mice supported the notion that the inhibition of γ-secretase caused toxicities by impacting Notch signaling. The skin cancer risk was predicted from animal studies in which γ-secretase/Notch signaling was globally attenuated [5, 6]. Thus, development of γ-secretase inhibitors/modulators that specifically inhibit the production of Aβ without influencing Notch activity is now necessary or mandatory as a treatment strategy for AD [2, 7].
In a recent paper, Basi and co-workers at Elan Pharmaceuticals report a preclinical study of two novel sulfonamide-type small molecules, ELN318463 and ELN475516 . These compounds showed predominant inhibitory potency against Aβ production over Notch signaling in vitro, suggesting that both molecules selectively inhibit APP cleavage by γ-secretase. Moreover, subchronic oral administration of one compound, ELN475516, significantly reduced Aβ levels in brains of APP transgenic as well as wild-type mice. Importantly, treatment with ELN475516 did not show any inhibition of the expression of Notch target genes at the peripheral target, and no overt systemic toxicity was observed. These data indicate that it is possible to develop compounds exhibiting APP-selective γ-secretase inhibition in vivo. Furthermore, Basi and colleagues revealed an intriguing finding regarding the molecular mechanism whereby these compounds confer APP selectivity by employing a chemical biology approach: only in the presence of the recombinant APP substrate were ELN compounds able to displace the active site isostere that directly targets both catalytic aspartates in PS1. In contrast, a non-selective classical inhibitor displaced the active site isostere from PS1 irrespective of the presence of the substrate, indicating that these ELN compounds selectively target the APP-cleaving PS1 conformation induced by the substrate. These data, therefore, suggest that APP-selective inhibition is feasible by developing small molecules that target the non-catalytic domains of γ-secretase. Supporting this notion, it has been reported that the substrate recognition mechanism in the γ-secretase is enzymatically distinct from the catalytic site, and that the substrate interaction induces an allosteric conformational change in the PS1 molecule . Interestingly, Basi and colleagues have reported previously that this class of compounds, known as 'Notch-sparing γ-secretase inhibitors' selectively inhibits PS1-containing over PS2-containing γ-secretase . Nevertheless, identification of the molecular target and the precise binding site of ELN compounds may provide important progress towards the rational development of APP-selective γ-secretase inhibitors. Future structural analysis of this atypical membrane-embedded protease would shed light on the mechanism of APP selectivity. In addition, molecular analysis of such selectivity of ELN compounds versus other γ-secretase substrates might further clarify the mechanism-based advantage of these compounds. Importantly, recent genetic and chemical biology approaches revealed that there are several possible druggable target molecules within the γ-secretase complex and the substrate [11–13]. In addition, after intensive efforts several laboratories have recently developed γ-secretase modulators that selectively inhibit Aβ42 production [14–16]. Combination approaches against several targets may be effective for reducing the levels of Aβ without inhibiting Notch in vivo . In conclusion, Basi and colleagues provide strong evidence that it is possible to develop small APP-selective γ-secretase inhibitors that 'spare' Notch. Understanding the precise molecular mechanism whereby ELN compounds modulate the γ-secretase activity would pave the way to develop a potentially safe and effective compound to attenuate β-amyloidosis in AD.
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The authors declare that they have no competing interests.
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Tomita, T., Wong, P.C. Selectivity to amyloid-β precursor protein cleavage provides hope against Alzheimer's. Alz Res Therapy 3, 7 (2011). https://doi.org/10.1186/alzrt66
- Notch Signaling
- Notch Target Gene
- Skin Cancer Risk
- Elan Pharmaceutical
- Strong Experimental Support