Fig. 6
From: Neuroinflammation increases oxygen extraction in a mouse model of Alzheimer’s disease
Immunofluorescence analysis of LPS’s effect on microglia and astrocyte activation in WT and AD mice. (A) Example coronal brain sections with the imaged field of view (around somatosensory cortex) indicated in red rectangle boxes. Image was captured with confocal tile imaging using 5X objective. Fluorescence signal is DAPI-labelled nuclei. (B) Example maximum intensity projection (MIP) of confocal images of WT and AD mice. Control groups are mice that did not receive LPS injection (n = 1 in each cohort). Green: astrocyte. Red: microglia. Blue: nuclei. Images displayed have the same intensity threshold. (C) Percentage area (PA) of Iba1 positive microglia and (D) GFAP positive astrocyte in different cortical layers and the entire imaged region of interest (ROI) around somatosensory cortex (denoted as ‘global’). Each mouse has two to six ROIs imaged from two to three micro-thickness brain slices. Filled black and red dots represent the result of a single ROI. Statistical difference between layers were tested using one-way ANOVA and Tukey’s post-hoc test. Difference between WT and AD was revealed by student’s t-test