Fig. 3

DesAb-O inhibits the binding of Aβ₄₂ oligomers to the neuronal membrane preventing their induced mitochondrial dysfunction. A,B Representative confocal microscopy images of SH-SY5Y cells treated with 3.0 μM (monomer equivalents) A + oligomers and ADDLs following 1 h pre-incubation in the absence or presence of DesAb-O (A) or A11 (B) Abs at the indicated Aβ₄₂:Abs molar ratios, where molar ratios refer to monomer equivalents. Red and green fluorescence indicates the cell membranes and Aβ₄₂ oligomers detected with the 6E10 Ab. C,D Degree of membrane binding of A + oligomers and ADDLs measured following incubation under the conditions represented in panels A and B, determined as reported in the Methods section. E MTT reduction in SH-SY5Y cells treated for 24 h with the indicated Aβ₄₂ aggregates at a concentration of 3.0 μM (monomer equivalents) following a 1 h pre-incubation in the absence or presence of the indicated Abs. Abs alone were also tested as a control. Experimental errors are S.E.M. (n = 4). Samples were analysed by one-way ANOVA followed by Bonferroni’s multiple-comparison test relative to untreated cells (*P < 0.05, **P < 0.01, and ***P < 0.001), or to cells treated with the same Aβ₄₂ species without any Ab (§P < 0.05, §§P < 0.01, §§§P < 0.001). 200–250 (A–D) and 250,000–300,000 (E) cells were analysed per condition