Fig. 8
From: Neuronal transcriptome, tau and synapse loss in Alzheimer’s knock-in mice require prion protein
Integration and cell-type classification of age-grouped snRNA-seq data sets. A Schematic of treatment group and cohort sample sizes by age group. B) Number of total nuclei per sample (left), mean reads per nuclei per sample (middle), and mean unique genes detected per nuclei per sample (right) from single-nucleus RNA-seq (snRNA-seq) of 10-month and 20-month cohorts. D Dot plot showing the percentage of nuclei and the scaled mean expression of cell-type classification markers used to identify the UMAP clusters in (C). E Comparative mean nuclei frequency of cluster cell types representing individual samples of WT, Prnp−/−, DKI, or DKI; Prnp−/− genotypes. Data presented as mean + SEM from individual samples by their respective genotype and compared by mixed model test with Tukey correction. ns = non-significant, *P < 0.0332, **P < 0.0021, *** < 0.0002,****P < 0.0001. F Nuclei proportions for neuronal and glial cell types across groups for 10-month and 20-month cohorts. G Nuclei proportions and UMAP projection of male and female representation within the age-group integrated snRNA-seq-data