Fig. 6

Prnp deletion does not alter Aβ accumulation in DKI mice but reduces periplaque dystrophic neurites. A Representative images of Thioflavin S (green) and D54D2 anti- Aβ antibody (red) staining from the hippocampus of 10-month-old WT, Prnp^−/−, DKI and DKI; Prnp^−/− mice. Scale bar = 100 μm. B Quantification of Thioflavin S stained area in hippocampus demonstrates a significant increase in dense core plaque load in DKI mice compared to WT, but no difference between DKI and DKI; Prnp^−/−. Data are graphed as mean ± SEM analyzed by ordinary one-way ANOVA with Tukey’s multiple comparisons test, **P < 0.01, ***P < 0.001, ****P < 0.0001, n = 9 for WT, n = 11 for Prnp^−/−, n = 9 for DKI, n = 12 for DKI; Prnp^−/−. C Quantification of D52D2 immunoreactive area in hippocampus documents a significant increase in Aβ levels in DKI mice compared to WT, but no difference between DKI and DKI; Prnp^−/−. Data are graphed as mean ± SEM, analyzed by ordinary one-way ANOVA with Tukey’s multiple comparisons test, ****P < 0.0001, n = 9 for WT, n = 11 for Prnp^−/−, n = 9 for DKI, and n = 12 for DKI; Prnp^−/−. D Representative images of anti-Lamp1 (red) immunostaining in medial cortex of 10-month-old WT, Prnp^−/−, DKI and DKI; Prnp^−/− mice. Scale bar = 100 μm. E Quantification of the area of Lamp1 immunoreactive accumulation in medial cortex demonstrates a significant increase of dystrophic neurites in DKI mice relative to WT. The accumulation of dystrophic neurites is less pronounced in DKI; Prnp^−/− mice than in DKI mice. Data are graphed as mean ± SEM, analyzed by ordinary one-way ANOVA with Tukey’s multiple comparisons test, *P < 0.05, ****P < 0.0001, n = 14 for WT, n = 17 for Prnp^−/−, n = 14 for DKI, and n = 18 for DKI; Prnp^−/−