Fig. 8
From: PACAP–Sirtuin3 alleviates cognitive impairment through autophagy in Alzheimer’s disease
Sirt3 knockdown abolished the effects of PACAP and reduced autophagy in HT22 cells. A Western blots and corresponding graphs show protein levels of Sirt3 after Sirt3 knockdown; n = 4. Western blots and corresponding graphs show protein levels of (B) Aβ (n = 4) and (C) total Tau (n = 5) after Sirt3 knockdown. Western blots and corresponding graphs show protein levels of (D and E) LC3-II:I, (F) p-PI3K:PI3K ratio, (G) p-AKT:AKT ratio, and (H) p-mTOR:mTOR ratio in the following treatment conditions: NC, Sirt3 knockdown, Sirt3 knockdown + PACAP treatment, Sirt3 knockdown + oligomeric Aβ exposure, and Sirt3 knockdown + oligomeric Aβ exposure + PACAP treatment, n = 4. *p < 0.05, **p < 0.01, ***p < 0.001