Fig. 3
From: PACAP–Sirtuin3 alleviates cognitive impairment through autophagy in Alzheimer’s disease
Expression levels of autophagy biomarkers in 3xTg mice were increased by PACAP treatment. Western blots and corresponding graphs show hippocampal protein levels of (A) LC3-II:I (n = 6), (B) ATG5 (n = 4), and (C) Beclin1 (n = 4). D Immunofluorescence staining of LC3A/B in the hippocampus of WT, 3xTg, and 3xTg + PACAP mice. Red color (RFP) represents LC3A/B; blue color (DAPI) represents cell nuclei. Scale bar, 75 μm; n = 3. E Quantitative analysis of MAP1LC3A/B fluorescence density. RT-qPCR analysis of mRNA levels of (F) ATG5 and (G) Beclin1 in the cortex of 3xTg mice, n = 4–5. *p < 0.05, **p < 0.01, ***p < 0.001