Fig. 4

Quantification of gap distance within the elastic fiber network at the media-adventitia interface. a, c, e, g Binarized multiphoton images of elastic fibers from Fig. 3e-h with region of interest selected. b, d, f, h The corresponding area fraction occupied by black pixels was plotted with the intima, media, and adventitia regions indicated. i The gap distance in the elastic fiber network at the media-adventitia interface was quantified and plotted for the control, early AD, intermediate AD, and advanced AD groups obtained from multiphoton image quantification. The top and bottom of the box presents the first and third quartile of the data set. The extended bars from top and the bottom of the box represent the maximum and minimum. The horizontal line in the box represents the median. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001