Fig. 3
FK506 rescues spine counts and reduces neuroinflammation. A Cortical golgi staining for spine counts. n = 3 mice per group. * = p < 0.05 by a one-way ANOVA with Tukey. Scale bar = 10 μm. B Average numbers of amyloid plaques per cortical section of APP/PS1 mice. n = 3 mice per group with 7–8 brain sections per mouse. * = p < 0.05 by a paired t-test. Scale bar = 500 μm. C β-amyloid 40 and 42 ratios in cortical lysates from APP/PS1 mice. FA99 = formic acid 99% extraction. FA = 70% extraction. GU = glutaraldehyde extraction. n = 3–4 mice per group. * = p < 0.05 by a two-tailed unpaired t-test. D Number of GFAP immunoreactive cells and Iba1 immunoreactive cells per μm.2 and distance from amyloid plaques in the APP/PS1 cortex. n = 3 biological replicates. For GFAP and Iba1 counts, 7–8 brain images were analyzed. For Iba1 distance from plaques, 25 plaques were analyzed per mouse and the number of Iba1 cells within 50 μm was counted. * = p < 0.05 by a two-tailed unpaired t-test. F Images used for quantification in D. Scale bar = 100 μm. For all graphs, WT (blue), WT + FK506 (green), APP/PS1 (red), and APP/PS1 + FK506 (purple)