Fig. 2
Pin1 and CN activity are normalized in FK506 treated mice. A CN activity assay. Data are means ± SEM; n = 3 biological replicates. * = p < 0.05 by one way ANOVA with Tukey. B Pin1 isomerase activity assay with brain lysates. Curves are normalized to WT activity 100%. n = 3 mice per group. * = p < 0.05 by a two-way ANOVA with Tukey. C Immunohistochemical analysis of pSer111 Pin1 in the hippocampus (CA1) of the experimental mice using anti-pSer111 antibody. D Quantification of the fold change of nuclear intensity for pSer111 Pin1. * = p < 0.05 by one-way ANOVA. E Quantification of percent of immunofluorescence present in the nucleus versus cell extensions for pSer111 Pin1. * = p < 0.05 by one-way ANOVA with Tukey. F Immunohistochemical analysis of total Pin1 in the hippocampus (CA1) of the experimental mice using total anti-Pin1 (rabbit) antibodies. G Quantification of the fold change of nuclear intensity for total Pin1. * = p < 0.05 by one-way ANOVA with Tukey. H Quantification of percent of immunofluorescence present in the nucleus versus cell extensions for total Pin1. * = p < 0.05 by one-way ANOVA with Tukey. I Double immunofluorescence with anti-pSer111 Pin1(rabbit) and total anti-Pin1 (mouse) in the CA1 of the experimental mice. J Ratio of pSer111 Pin1/total Pin1 in the nucleus of the CA1. * = p < 0.05 by one-way ANOVA with Tukey. K Ratio of pSer111 Pin1/total Pin1 in the apical projections of the CA1. * = p < 0.05 by one-way ANOVA with Tukey. Scale bar = 20 μm. For all graphs, WT (blue), WT + FK506 (green), APP/PS1 (red), and APP/PS1 + FK506 (purple)