Fig. 4
Flow cytometry and immunofluorescence analysis revealed considerable differences in the inflammatory response comparing WT and AppNL−G−F mice. A ECB-adhering cells at post-explantation were collected, pooled, and surface stained for various immune cell markers and analyzed by flow cytometry. Pan-leukocyte marker CD45 was used to select immune cells and was sub-gated to identify specific cell types including T-, natural killer (NK)-, B-, dendritic (DC)-, and microglia-macrophage (CD11b) cells, respectively. B Representative immunohistochemical images showing BDNF status, microglia (Iba1), astrocytes (GFAP), leukocyte (CD45), and fibroblasts in the proximity (P) and distal (D) area surrounding the ECB-BDNF devices after 1 month of treatment (n = 2–4). Scale bar 300 μm