Table 1 Demographics and biomarkers levels

Age, years

64.6

(9.42)

66.30 (9.58)

66.04 (7.73)

72.40 (7.96)^a

71.90 (8.61)^a

< 0.0001

70.4

(5.8)

71.4

(6.1)

70.0

(6.1)

70.1

(4.5)

74.4

(4.8)

73.5

(4.5)

0.009

Male (% male)

8

(38%)

16

(34%)

15

(56%)

16

(40%)

30

(39%)

0.338

3

(27%)

16

(57%)

6

(32%)

2

(29%)

19

(41%)

25

(39%)

0.141

APOEε4 carriers

7/21

(33%)

5/47

(11%)^a

7/26

(27%)

22/40

(55%)

51/76

(67%)^a

< 0.001

1/10

(10%)

4/24

(17%)

3/18

(17%)

3/7

(43%)

24/38 (63%)^a

39/62 (61%)^a

< 0.02

Level of education, years

11.5 (2.4)

10.7 (2.2)

11.0 (2.1)

10.0 (2.5)

10.6 (2.4)

0.299

10.4 (4.9)

8.5 (4.6)

7.75 (4.7)

11.4 (4.0)

8.4 (4.6)

7.7 (3.6)

0.0267

MMSE score

27.14 (2.45)

24.00 (4.01)^a

23.85 (4.87)^a

23.82 (3.84)^a

19.09 (5.74)^a

< 0.0001

28.4

(2.3)

25.6

(2.7)

22.2

(5.8)^a

28.7

(1.5)

23.3

(3.9)^a

20.4

(4.8)^a

< 0.0001

Core biomarkers Lumipulse

 CSF Aβ_1–42

1060.95

(245.23)

1090.66 (468.75)

1027.78 (354.02)

553.00 (209.36)^a

497.88 (155.27)^a

< 0.0001

1186

(357)

1164

(549)

986

(449)

634

(206)^a

556

(178)^a

522

(153)^a

< 0.0001

 CSF Aβ_1–42/40

0.093

(0.010)

0.088 (0.012)

0.09

(0.01)

0.044 (0.009)^a

0.042 (0.009)^a

< 0.0001

0.091 (0.017)

0.0879 (0.012)

0.091 (0.017)

0.049 (0.008)^a

0.043 (0.009)^a

0.043 (0.008)^a

< 0.0001

 CSF pTau, pg/mL

32.25

(8.16)

40.97 (20.81)

33.59

(10.50)

94.80 (47.31)^a

113.53 (60.02)^a

< 0.0001

56.8

(31.4)

50.2

(30.9)

41.0

(24.4)

59.1

(14.3)

112

(59.6)^a

121.4 (57.9)^a

< 0.0001

 CSF t-tau, pg/mL

236.62

(63.69)

333,085 (172.630)

362.26

(341.90)

594.83 (285.45)^a

722.01 (393.87)^a

< 0.0001

307

(141)

357

(230)

311

(166)

407

(107)

669

(323)^a

759

(342)^a

< 0.0001

Biomarker profile

 Normal profile

21 (100%)

39 (83%)

25 (92%)

0 (0%)

0 (0%)

9 (82%%)

24 (86%)

17 (89%)

0 (0%)

0 (0%)

0 (0%)

 AD continuum

0 (0%)

0 (0%)

0 (0%)

40 (100%)

77 (100%)

0 (0%)

0 (0%)

0 (0%)

7 (100%)

46 (100%)

64 (100%)

 A+T−

/

/

/

7 (18%)

10 (13%)

/

/

/

6 (86%)

11

(24%)

3

(5%)

 A+T+

/

/

/

33 (82%)

67 (87%)

/

/

/

1

(14%)

35

(76%)

61

(95%)

 SNAP

0

(0%)

8 (17%)

2 (8%)

0

(0%)

0

(0%)

2 (18%)

4 (14%)

2 (11%)

0 (0%)

0 (0%)

0 (0%)

CSF p-tau biomarkers

 CSF N-term p-tau181, pg/mL

224.72

(66.33)

300.708 (245.52)

242.23 (110.58)

965.91 (628.08) ^a

1223.94 (802.66)^a

< 0.0001

427

(110)

410

(164)

426

(168)

494

(129)

771

(257)^a

871

(343)^a

< 0.0001

 CSF N-term p-tau217, pg/mL

1.84

(0.94)

2.90

(2.50)

2.14

(1.67)

13.88 (9.48)^a

16.62 (9.74)^a

< 0.0001

N/A

N/A

N/A

N/A

N/A

N/A

N/A

 CSF N-term p-tau231, pg/mL

271.93

(64.74)

325.05 (148.44)

286.62 (114.72)

657.64 (205.53)^a

800.58 (640.95)^a

< 0.0001

313

(211)

348

(201)

331

(236)

541

(144)

788

(399)^a

893

(469)^a

< 0.0001

 CSF N-term p-tau235, pg/mL

11.19

(2.94)

12.74 (5.67)

11.56 (4.30)

24.75 (8.11)^a

28.03 (11.52)^a

< 0.0001

17.6

(8.10)

18.5

(11.3)

17.4

(7.23)

23.1

(3.65)

34.8

(12.9)^a

41.6

(20.0)^a

< 0.0001