Fig. 5

Activation and differentiation of microglia in the hippocampus and cortical areas in mouse brains. A, B Representative immunofluorescence images of microglial activation for Iba-1 and CD68 staining in the cortex and hippocampus. C, H Representative images of CD206, CD68, CD16/32, and Iba-1 detected by Western blot in the cortex and hippocampus. D–G In the cortex, the CD68, CD16/32, CD206, and Iba-1 levels were significantly higher in AD + Saline mice than in WT + Saline mice. The CD68, CD16/32, and Iba-1 levels were significantly higher in the AD + Poly(I:C) mice than in the WT + Saline mice. Compared with those of the AD + Saline mice, the CD206 levels were significantly lower. I–L In the hippocampus, the CD68, CD16/32, CD206, and Iba-1 levels were significantly higher in the AD + Saline mice than in the WT + Saline mice and were significantly lower in the AD + Poly(I:C) mice than in AD + Saline mice (#: WT + Saline vs. AD + Saline, P < 0.05; &: WT + Saline vs. AD + Poly(I:C), P < 0.05; *: AD + Saline vs. AD + Poly(I:C), P < 0.05; n = 4/group)