Fig. 4

YIAD002 disrupts Aβ aggregation by blocking intermolecular folding of paired β-strands. A Scheme of mapping assay using partial fragments of Aβ. B Mapping assay indicates region of Aβ dissociation by YIAD002: Aβ(16–21) in purple and Aβ(32–37) in cyan. C Docking models of YIAD002 interaction with Aβ(1–42). D Dissociative effects of YIAD002 against Aβ(pE3-42) aggregation were evaluated using ThT fluorescence. Aggregation for Aβ(pE3-42) is indicated as “+” for 1 day, and “++” for 4 days. Fluorescence intensities were normalized and statistically compared to the Aβ(pE3-42)-only 1 day (+) control (100%). One-way analysis of variance followed by Bonferroni’s post hoc comparisons tests were performed in all statistical analyses (****P < 0.0001). Data are presented as mean ± SEM. Conc. = concentration, Aggr. = aggregation, Cpd. = compound