Fig. 5

Gossypetin increases MHC II⁺ microglia. A, B Representative images of primary microglia stained with Hoechst, Iba-1, and MHC II. Primary microglia were pretreated with gossypetin (25 μM) for 24 h and treated with recombinant Aβ (1 μM). Scale bar corresponds to 50μm (A). Bar graph represents quantification of MHC II⁺ microglia percentage (n = 6~7, 88~203 cells per set) (B). C, D Representative images of hippocampus stained with Hoechst, Iba-1, and MHC II in 5xFAD and gossypetin treated 5xFAD mice. Scale bar corresponds to 50μm (C). Average number of MHC II⁺ microglia were counted (n = 6 per group, 3 slices per brain) (D). E, F Representative images of cortex stained with Hoechst, Iba-1, and MHC II in 5xFAD treated with vehicle or gossypetin. Scale bar corresponds to 50μm (E). Bar graph represents average number of MHC II⁺ microglia that were counted (n = 6 per group, 3 slices per brain) (F). The error bars represent the mean ± SEM. ****p < 0.0001, **p < 0.01, *p < 0.05, ns = not significant, two-way ANOVA followed by Tukey’s multiple comparisons test