Fig. 3
From: Gossypetin ameliorates 5xFAD spatial learning and memory through enhanced phagocytosis against Aβ
Single-cell characterization of altered transcriptome profiles of 5xFAD microglia by gossypetin administration. (A) Uniform manifold approximation and projection (UMAP) plots showing all microglial cells from hippocampus region. The cells are colored by their cell types. (B) Heatmap showing the Z-scores of average normalized expressions of representative DEGs for each cell type from hippocampus region. (C) Scatter plot showing biological processes of Gene Ontology (GOBP) terms that are upregulated or downregulated by 5xFAD or gossypetin administration for each microglial subtype from hippocampus. Among the significant (Fisher’s exact test, p < 0.01) terms, terms associated with antigen presentation and phagocytosis are colored by their biological keywords. (D) Bar plot displaying gene set enrichment analysis (GSEA)-calculated normalized enrichment score (NES) of GOBP terms for gossypetin administration condition against vehicle treatment within 5xFAD homeostatic microglia from hippocampus. (E) Volcano plot illustrating the differentially expressed genes selected by the comparison between wild type and 5xFAD (left-top) or vehicle and gossypetin (left-bottom, and right) cells from homeostatic microglia and disease-associated microglial population of hippocampus region. Significant (p < 0.05) and DAM-related genes are labeled