Fig. 8
Detection of GABAB1-GIRK2 heterodimers in the hippocampus of wild type and APP/PS1. A, B Photomicrographs of nuclei staining (DAPI) and dual recognition in green of GABAB1 and GIRK2 with the proximity ligation in situ assay (P-LISA), in the stratum radiatum of CA1 area of WT and APP/PS1 mice. The interaction between GABAB1 and GIRK2 is observed by the green dots. C Quantification of P-LISA signals for GABAB1 and GIRK2 proximity confirmed the significant reduction in the density of dots/μm2 in APP/PS1 mice compared to age-match wild type (**p<0.01, t-test). Values correspond to the mean ± SEM. Scale bars: 20 μm