Fig. 9
ISAD1 and ISAD1rev prevent toxic effects of Tau aggregates. 200 μM of TauRDΔK was incubated with or without peptides (1:10) respectively for 24 h at 37 °C. Pre-incubated samples (10 μM Tau, 100 μM peptide final concentration) were added to N2a-TauRDΔK cells followed by recording of MTT, LDH, and ROS intensities. A Pre-incubated Tau with peptides inhibit Tau toxic effects by preserving cell viability (blue and red bars) [n = 3; one-way ANOVA with Tukey’s post hoc test; F(5, 48) = 102; ***p ≤ 0.001]. B Low LDH release levels show protection of cell membrane integrity by peptides (blue and red bars) [n = 3; one-way ANOVA with Tukey’s post hoc test; F(5, 48) = 268; ***p ≤ 0.001]. C Within 30 min, the cells increase ROS level in the aggregate-treated cells (light blue bar). Pre-incubated Tau with peptides inhibit Tau toxic effects in reducing intracellular ROS levels (blue and red bars) compared to positive control TBHP (beige bar) [n = 3; one-way ANOVA with Tukey’s post hoc test; F(23, 192) = 209; ***p ≤ 0.001]