Fig. 8
From: The apolipoprotein receptor LRP3 compromises APP levels
Chloroquine increases full-length APP and sAPP levels at the membrane fraction. Western blots and quantification of the expression of full-length APP (fAPP), APP-CTF, Flag (LRP3-flag), and tubulin in cytosol and plasma membrane-containing fractions (plasma membrane f. or plasma mb f.) and in intracellular membrane-containing fractions (intracell membrane f. or intracell mb f.) from CHO-PS70 cell homogenates. The presence of LRP3-flag was only observed in the cytosol and plasma membrane fraction, and tubulin was not present in the intracellular membrane-containing fraction. For LC3B-I conversion to LC3B-II to monitor autophagy, the whole cell extracts were used. a Comparison between CHO-PS70 cells transfected with LRP3-flag (CHO LRP3) or pcDNA3.1 (CHO control). b Comparison between CHO cells transfected with pcDNA3.1 (CHO control) for 24h and then treated with 10 mM chloroquine (CQ) for another 24 h or with vehicle (veh, Hank’s media). c Comparison between CHO cells transfected with LRP3-flag (CHO LRP3) for 24h and then treated with 10 mM chloroquine (CQ) for another 24 h or with vehicle (veh, Hank’s media). (n = 6–10 for each condition, **p<0.01; *p<0.05 t-test)