Fig. 2

ApoER2-ICD increases LRP3 expression. a Representative western blot showing the expression of ApoER2-ICD after transfection with full-length ApoER2 cDNA (ApoER2) and reelin treatment (12μg/ml, ApoER2 + reelin) for 24 h in SH-SY5Y cells. For comparison, the expression of the ApoER2-ICD construct (ApoER2-ICD) is also shown. b Western blot and c quantification of LRP3 protein expression after ApoER2-ICD transfection in SH-SY5Y cells. Tubulin was used as an internal control (n = 6 for each condition, *p < 0.001, t-test). d qRT-PCR analysis showing the expression of LRP3 mRNA (n = 7 for each condition, *p < 0.05, t-test) and e LDLR mRNA (n = 10 for each condition) after transfection with GFP cDNA (control) and ApoER2-ICD cDNA in SH-SY5Y cells. Note that the X axis in d begins at 50%. 18S was used as an internal control for mRNA expression