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Fig. 3 | Alzheimer's Research & Therapy

Fig. 3

From: N-Methyl-D-aspartate (NMDA) and cannabinoid CB2 receptors form functional complexes in cells of the central nervous system: insights into the therapeutic potential of neuronal and microglial NMDA receptors

Fig. 3

DMR and intracellular calcium mobilization in HEK-293T cells expressing NMDA-CB2R heteromers. HEK-293T cells were transfected with the cDNAs for two protomers of the NMDA receptor: GluN1 (1 μg) and GluN2B (0.75 μg) and/or with the cDNA for the CB2R (1 μg); for calcium assays, cells were also transfected with the cDNA for the engineered calcium sensor, 6GCaMP (1 μg) (D–F). Receptors were activated using selective agonists (40 μM NMDA (for calcium detection assays) or 15 μM NDMA (for DMR assays) for NMDAR and/or 100 nM JWH-133 for CB2R). When indicated cells were pretreated with selective receptor antagonists (1 μM MK-801 for NMDA or 1 μM SR-144528 for CB2R). Real-time calcium-induced fluorescence (A-C) or DMR readings (D-F) were collected. Values in each figure are from a representative experiment out of 5 independently performed

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Alzheimer's Research & Therapy

ISSN: 1758-9193