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Table 8 Dilutional linearity of endogenous GFAP in plasma samples

From: Development of a novel, sensitive translational immunoassay to detect plasma glial fibrillary acidic protein (GFAP) after murine traumatic brain injury

Dilution factor

Mean duplicate CV (%)

Mean CV from neat specimen (%)

%recovery*

(mean ± SD) (n = 3)

1:1

2.7

 –

–

1:2

3.8

2.6

96.5 ± 4.7

1:4

1.6

2.6

98.2 ± 4.2

1:8

2.9

3.9

96.6 ± 5.6

1:16

0.8

3.0

104.4 ± 1.3

1:32

1.4

7.8

111.8 ± 6.7

1:64

6.2

18.2

131.2 ± 23.9

  1. *%recovery = (measured concentration adjusted for dilution/measured concentration of undiluted sample) × 100%. CV coefficient of variation, SD standard deviation
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Alzheimer's Research & Therapy

ISSN: 1758-9193