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Table 8 Dilutional linearity of endogenous GFAP in plasma samples

From: Development of a novel, sensitive translational immunoassay to detect plasma glial fibrillary acidic protein (GFAP) after murine traumatic brain injury

Dilution factor Mean duplicate CV (%) Mean CV from neat specimen (%) %recovery*
(mean ± SD) (n = 3)
1:1 2.7  –
1:2 3.8 2.6 96.5 ± 4.7
1:4 1.6 2.6 98.2 ± 4.2
1:8 2.9 3.9 96.6 ± 5.6
1:16 0.8 3.0 104.4 ± 1.3
1:32 1.4 7.8 111.8 ± 6.7
1:64 6.2 18.2 131.2 ± 23.9
  1. *%recovery = (measured concentration adjusted for dilution/measured concentration of undiluted sample) × 100%. CV coefficient of variation, SD standard deviation