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Table 6 Limits of quantification and detection

From: Development of a novel, sensitive translational immunoassay to detect plasma glial fibrillary acidic protein (GFAP) after murine traumatic brain injury

Measured LLOQ Measured ULOQ Calculated LLOQ Calculated LLOD
24.8 pg/mL* 16,533.9 pg/mL* 23.7 pg/mL# 9.0 pg/mL
  1. *All sample replicates < 20% CV; therefore, actual LLOQ may be lower and ULOQ may be higher than reported here. #calculated as 10 SD above blank measurement from 16 blank replicates. calculated as 2.5 SD above blank measurement from 16 blank replicates. LLOQ lower limit of quantification, ULOQ upper limit of quantification, LLOD lower limit of detection, CV coefficient of variation, SD standard deviation