|
Plasma pool
|
Mean concentration (pg/mL)
|
Group variation
|
Intra-assay variation
|
Inter-assay variation
|
|---|
|
SE (pg/mL)
|
Variance (pg/mL)
|
SD (pg/mL)
|
SE (pg/mL)
|
%CV
|
Variance (pg/mL)
|
SD (pg/mL)
|
SE (pg/mL)
|
%CV
|
|---|
|
All (model 1A)
|
1720.0
|
93.5
|
3484.7
|
59.0
|
26.4
|
3.4
|
17,763.2
|
133.3
|
59.6
|
7.7
|
|
Low (model 2B)
|
43.4
|
–
|
4.0
|
2.0
|
0.9
|
4.6
|
3.5
|
1.9
|
0.8
|
4.3
|
|
Intermediate (model 3C)
|
136.6
|
–
|
23.5
|
4.8
|
2.2
|
3.5
|
24.2
|
4.9
|
2.2
|
3.6
|
|
High (model 4D)
|
4979.9
|
–
|
0.0
|
0.0
|
0.0
|
0.0
|
70,844.3
|
266.2
|
119.0
|
5.3
|
- AAll groups: plasma pool = fixed effect, technical replicate = random effect nested in assay date, assay date = random effect. BLow plasma pool: technical replicate = random effect nested in assay date, assay date = random effect. CIntermediate plasma pool: technical replicate = random effect nested in assay date, assay date = random effect. DHigh plasma pool: technical replicate = random effect nested in assay date, assay date = random effect. SD for intra-assay and inter-assay variation were calculated as follows: SD= √variance). SE for intra-assay and inter-assay variation were calculated as follows: SE = SD/(√n), n = 5. SE standard error, SD standard deviation, CV coefficient of variation
