Development of a novel, sensitive translational immunoassay to detect plasma glial fibrillary acidic protein (GFAP) after murine traumatic brain injury

Table 5 Mixed model analysis estimates of fixed effects and covariance

Plasma pool	Mean concentration (pg/mL)	Group variation	Intra-assay variation				Inter-assay variation
Plasma pool	Mean concentration (pg/mL)	SE (pg/mL)	Variance (pg/mL)	SD (pg/mL)	SE (pg/mL)	%CV	Variance (pg/mL)	SD (pg/mL)	SE (pg/mL)	%CV
All (model 1^A)	1720.0	93.5	3484.7	59.0	26.4	3.4	17,763.2	133.3	59.6	7.7
Low (model 2^B)	43.4	–	4.0	2.0	0.9	4.6	3.5	1.9	0.8	4.3
Intermediate (model 3^C)	136.6	–	23.5	4.8	2.2	3.5	24.2	4.9	2.2	3.6
High (model 4^D)	4979.9	–	0.0	0.0	0.0	0.0	70,844.3	266.2	119.0	5.3

^AAll groups: plasma pool = fixed effect, technical replicate = random effect nested in assay date, assay date = random effect. ^BLow plasma pool: technical replicate = random effect nested in assay date, assay date = random effect. ^CIntermediate plasma pool: technical replicate = random effect nested in assay date, assay date = random effect. ^DHigh plasma pool: technical replicate = random effect nested in assay date, assay date = random effect. SD for intra-assay and inter-assay variation were calculated as follows: SD= √variance). SE for intra-assay and inter-assay variation were calculated as follows: SE = SD/(√n), n = 5. SE standard error, SD standard deviation, CV coefficient of variation

ISSN: 1758-9193