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Table 12 Impact of blood collection and processing method on assay performance

From: Development of a novel, sensitive translational immunoassay to detect plasma glial fibrillary acidic protein (GFAP) after murine traumatic brain injury

Sample handling Mean duplicate CV (%) %recovery*
(mean ± SD) (n = 3)
  Sham mice TBI mice Sham mice TBI mice
Cardiac puncture, centrifuged < 1 h after collection 7.8 4.7
Saphenous collection 5.1 9.4 90.3 ± 44.3 163.4 ± 1.8
4 h delay before centrifugation 5.4 3.3 100.5 ± 15.4 111.5 ± 1.5
  1. *%recovery = (concentration measured with modified collection or processing/concentration measured with optimal collection and processing) × 100%. CV coefficient of variation, SD standard deviation