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Table 12 Impact of blood collection and processing method on assay performance

From: Development of a novel, sensitive translational immunoassay to detect plasma glial fibrillary acidic protein (GFAP) after murine traumatic brain injury

Sample handling

Mean duplicate CV (%)

%recovery*

(mean ± SD) (n = 3)

 

Sham mice

TBI mice

Sham mice

TBI mice

Cardiac puncture, centrifuged < 1 h after collection

7.8

4.7

Saphenous collection

5.1

9.4

90.3 ± 44.3

163.4 ± 1.8

4 h delay before centrifugation

5.4

3.3

100.5 ± 15.4

111.5 ± 1.5

  1. *%recovery = (concentration measured with modified collection or processing/concentration measured with optimal collection and processing) × 100%. CV coefficient of variation, SD standard deviation