Fig. 6

Immunohistochemical analysis of Aβ aggregates in hippocampal brain sections from 5xFAD mice treated with K11_IgG2a, 3D6_IgG2a, and isotype control. a Immunohistochemical analysis. Three-month-old 5xFAD mice were treated intraperitoneally once a week with 12 mg/kg K11_IgG2a, 3D6_IgG2a, or isotype control. After 38 weeks of treatment, mice were sacrificed. The right hemisphere was treated with paraformaldehyde, cryoconserved, and sliced. ROI in hippocampal brain slices were selected by staining with anti-isoD7-Aβ antibody K16 or total Aβ antibody 3D6 as indicated, followed by application of biotinylated anti-mouse IgG2b or anti-mouse IgG1, respectively. b Quantitative evaluation. Area of isoD7- or total Aβ containing peptides (ROI in %) was quantified based on overall area of ROI by using the program BZ II Analyzer. For statistical analysis, Tukey’s multiple comparison test was used. Sample size was 3 slices per brain and at least 10 animals per group. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. The error bars represent SEM