Fig. 4

Analysis of Aβ deposits in brain samples from 5xFAD mice treated with K11_IgG2a and isotype control. Three-month-old 5xFAD mice were treated intraperitoneally once a week with 500 μg, 150 μg K11_IgG2a, or 500 μg isotype control. After 12 weeks of treatment, mice were sacrificed. a Immunohistochemical analysis—the right hemisphere was treated with paraformaldehyde and slices were prepared. Brain slices have been incubated with antibody 6E10, followed by application of biotinylated anti mouse IgG1. b Quantification of total Aβ and isoD7-Aβ peptides—the left hemisphere was homogenized in T-Per buffer, followed by centrifugation. The pellet was resuspended in 5 M GdmCl, again centrifuged and the supernatants applied to a total Aβ (left) and isoD7-Aβ (right) specific ELISA. Sample size was at least 6 animals per group. The error bars represent SEM