Fig. 5

Aβ40 mutants are more protective than Aβ40 WT. a Panels show SEM of flies co-expressing Aβ42 WT and mutant Aβ40 and Aβ42. Interestingly, co-expressing Aβ42 WT with Aβ40 WT, Aβ40 E22G had no effect on the overall eye phenotype, showing similar size and degree of organization in the ommatidia when compared to flies expressing Aβ42 WT alone. However, flies co-expressing Aβ42 WT with Aβ40 S26C and Aβ40 E22Q showed mild improvement on ommatidia organization at the anterior part of the eye. Flies co-expressing Aβ42 together with Aβ42 mutants (E22G, S26C, and E22Q) produced a more severe phenotype with small and disorganized eyes, fused ommatidia, and frequent appearance of necrotic spots. b Box-whisker graph representing the phenotypical scores provided by Flynotyper software of flies co-expressing Aβ42 WT and Aβ40 with mutations. Data shows median, quartiles, and mean percentage of SEM flies images (n = 3). Flies expressing Aβ42 WT + LacZ (black), Aβ42 WT + Aβ40 WT (red), Aβ42 WT + Aβ40 E22G (brown), Aβ42 WT + Aβ40 S26C (blue), and Aβ42 WT + Aβ40 E22Q (green) were analyzed. A lower phenotypic score indicates a decrease in disorganization of the ommatidial arrangement, which correlates with a decrease in severity of the eye phenotype. c Box-whisker graph representing the phenotypical scores provided by Flynotyper software of flies co-expressing Aβ42 WT and Aβ42 with mutations. Data shows median, quartiles, and mean percentage of SEM fly images (n = 3). Flies expressing Aβ42 WT + LacZ (black), Aβ42 WT + Aβ42 WT (red), Aβ42 WT + Aβ42 E22G (brown), Aβ42 WT + Aβ42 S26C (blue), and Aβ42 WT + Aβ42 E22Q (green) were analyzed. A higher phenotypic score indicates an increase in disorganization of the ommatidial arrangement, which correlates with an increase in severity of the eye phenotype. ANOVA, *p < 0.01, **p < 0.001, ***p < 0.0001