Fig. 5

AQP4 deletion increased intraneuronal apoE aggregation in 3-month-old AQP4^−/−/APP/PS1 mice. a, b Representative bands of Western blot and densitometry analysis of apoE and LRP1. c Immunofluorescence of apoE. ApoE expression was significantly decreased in the cerebral cortex following local injection of AAV encoding apoE siRNAs. d Quantification of apoE-positive area fraction in the cerebral cortex. e Double immunofluorescence of NeuN and total-Aβ. Total-Aβ expression was also obviously decreased in the cerebral cortex following local injection of AAV encoding apoE siRNAs. f, g Quantification of total-Aβ and NeuN/total-Aβ-positive area fraction in the cerebral cortex, respectively. h, i Representative bands of Western blot and densitometry analysis of APP, CTF-β, and Aβ monomers/oligomers in the cortex after local injection of AAV encoding apoE siRNAs. Data were analyzed by two-way ANOVA with Newman-Keuls post hoc test. Data are mean ± SEM, n = 4 per group, *p < 0.05; ***p < 0.001