Fig. 2

Different concentrations of ZGM1 have different effects on Aβ aggregation. a Aβ42 aggregation monitored by thioflavin T (ThT) fluorescence. Aβ (10 μM) was incubated with 20 μM ThT and the corresponding ratio of ZGM1 at 37 °C. The error bars represent the S.D. b, c Analysis of Aβ42 (50 μM) aggregates by TEM. Samples were incubated with the corresponding ratio of ZGM1 for 24 h (b) or for 7 days (c) at 37 °C. Scale bars, 100 nm. d, e Dot blotting of total Aβ (anti-Aβ: 6E10, also recognizes APP) and Aβ oligomers (anti-amyloidogenic protein oligomers: A11). d Aβ42 was incubated without or with ZGM1 for 0, 1, 3, or 5 days at 37 °C. e Aβ42 was incubated without or with ZGM1 (5 μM, 50 μM) for 5 days at 37 °C