Fig. 2

Beta amyloid expression in HHV-6-infected microglial cells. a Expression of the two Abeta isoforms more associated to AD (Aβ1-40 and Aβ1-42) was evaluated in monolayer microglial cells infected at a multiplicity of infection of 100 genome equivalent/cell at 1, 3, 7, and 14 d.p.i. The results are reported as percentage of the isoform on the total expression. b HHV-6A-infected microglial cells (m.o.i. 100:1; 3, 14 d.p.i.) were stained with Hoechst, anti-gp116 FITC, and anti Abeta 1-42 PE moAbs. Images were taken in fluorescence (Nikon Eclipse TE2000S) equipped with a digital camera. Original magnification × 20. Spheroid 3D models obtained with PBM-microglial cells from healthy subjects. c Microglial cells were infected at a multiplicity of infection of 100 genome equivalent/cell. Virus transcription (RNA) was evaluated by RT-qPCR performed on U42 virus gene, at 1, 3, 7, and 14 d.p.i., as already detailed. Results are expressed in log target molecules referred to duplicates of 2 independent assays. d Expression of Aβ1-42 and gp116 was evaluated in spheroid 3D microglial cells infected at a multiplicity of infection of 100 genome equivalent/cell at 1, 3, 7, and 14 d.p.i. The results are reported as percentage of the isoform on the total expression. e HHV-6A-infected spheroid 3D microglial cells (m.o.i. 100:1; 3; 14 d.p.i.) [14] were stained with Hoechst, anti-gp116 FITC, and anti Abeta 1-42 PE moAbs. Images were taken in fluorescence (Nikon Eclipse TE2000S) equipped with a digital camera. Original magnification: × 20, left panels; × 40, right panels