Table 1 Effect of rHDL-rApoJ nanodiscs or free rApoJ on brain inflammatory status, analyzed by multiplexed ELISA. Data are expressed as the mean ± SEM. N = 5–7/group. *p < 0.05 and **p < 0.01 refer to statistical differences vs. the saline-treated group determined by one-way ANOVA test (plus multiple comparison test). The false discovery rate (FDR) correction was used to control for multiple testing in the multiplexed analysis of brain inflammatory status
Inflammatory markers | APP23 saline (pg/mg) | APP23 rHDL-rApoJ (pg/mg) | APP23 free rApoJ (pg/mg) | One-way ANOVA (3 groups) p value | rHDL-rApoJ vs saline FDR-corrected p value | Free rApoJ vs saline FDR-corrected p value |
|---|---|---|---|---|---|---|
FGF-basic | 984.4 ± 154.6 | 895.6 ± 113.3 | 560.2 ± 151.4 | p = 0.108 | p = 0.787 | p = 0.174 |
IL-1β | 85.28 ± 12.17 | 50.86 ± 3.75 | 72.91 ± 11.43 | p = 0.080 | p = 0.122 | p = 0.448 |
IL2 | 151.0 ± 13.09 | 116.7 ± 14.22 | 125.2 ± 14.49 | p = 0.222 | p = 0.198 | p = 0.324 |
IL5 | 141.7 ± 26.35 | 130.61 ± 27.54 | 148.0 ± 21.31 | p = 0.893 | p = 0.819 | p = 0.874 |
IL6 | 203.1 ± 22.15 | 177.7 ± 28.82 | 176.3 ± 19.5 | p = 0.656 | p = 0.583 | p = 0.448 |
IL13 | 21.31 ± 3.40 | 17.68 ± 2.34 | 12.63 ± 3.22 | p = 0.171 | p = 0.552 | p = 0.183 |
IL17 | 20.01 ± 2.33 | 13.9 ± 1.37* | 10.23 ± 1.08** | p = 0.004 | p = 0.122 | p = 0.017 |
IFN-γ | 127.8 ± 15.53 | 89.81 ± 13.28 | 94.08 ± 10.68 | p = 0.123 | p = 0.137 | p = 0.194 |
IP10 | 54.4 ± 4.34 | 44.48 ± 5.11 | 46.71 ± 5.05 | p = 0.330 | p = 0.289 | p = 0.388 |
KC | 1558 ± 115.5 | 1206 ± 112.0* | 1043 ± 104.6** | p = 0.012 | p = 0.122 | p = 0.029 |
MIG | 81.65 ± 8.21 | 57.81 ± 6.91 | 61.48 ± 8.72 | p = 0.090 | p = 0.122 | p = 0.194 |
TNF-α | 127.2 ± 13.46 | 125.2 ± 9.43 | 144.7 ± 17.79 | p = 0.620 | p = 0.937 | p = 0.448 |
VEGF | 17.67 ± 2.36 | 14.27 ± 2.35 | 10.72 ± 1.22 | p = 0.080 | p = 0.399 | p = 0.129 |
MCP-1 | 36.1 ± 3.93 | 24.31 ± 2.84 | 28.19 ± 3.12 | p = 0.060 | p = 0.122 | p = 0.194 |