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Table 3 Antibody dose-dependent prevention of amyloid accumulation and inflammation in APPSL transgenic mice

From: SAR228810: an antibody for protofibrillar amyloid β peptide designed to reduce the risk of amyloid-related imaging abnormalities (ARIA)

Treated groups Ctrl-IgG1 SAR255952 3D6
Dose (mg/kg) 10 1 3 10 3 10
Biochemical cortical Aβ
 Pan-Aβ μg/g tissue 23.7 ± 1.7 25.1 ± 1.8 17.8 ± 1.7 14.0 ± 1.4 24.3 ± 2.3 13.5 ± 3.4
 % reduction vs. Ctrl-IgG1   +6% –25% −41% +3% −43%
NS NS p < 0.05 NS p < 0.001
 Aβ42 μg/g tissue 7.62 ± 0.58 8.86 ± 0.74 6.62 ± 0.68 5.28 ± 0.77 8.49 ± 0.84 4.92 ± 1.65
 % reduction vs. Ctrl-IgG1   +16% −13% −31% +11% −35%
NS NS NS NS NS
 Cystatin F mRNA hippocampus 4.47 ± 0.35 4.27 ± 0.35 2.35 ± 0.29 0.99 ± 0.07 4.99 ± 0.37 2.19 ± 0.49
 % reduction vs. Ctrl-IgG1   −4% −47% −78% +12% −51%
NS p < 0.05 p < 0.001 NS p < 0.05
Aβ immunohistochemistry
 Total area cortex (μm2) 382,388 339,950 188,729 83,495 387,344 151,764
 SEM 29,945 44,091 19,154 14,776 53,255 31,968
 % reduction vs. Ctrl-IgG1   −11% −51% −78% +1% −60%
NS p = 0.013 p < 0.0001 NS p = 0.003
 Total area hippocampus (μm2) 103,417 66,849 42,794 20,646 77,838 60,972
 SEM 11,020 13,435 4546 4329 9786 8192
 % reduction vs. Ctrl-IgG1   −35% −59% −80% −25% −41%
NS p = 0.0123 p < 0.0001 NS p = 0.0116
Plasma Aβ40 pg/ml 175 ± 31 198 ± 20 201 ± 34 211 ± 27 680 ± 130 999 ± 142
  1. For the different parameters, values represent mean ± standard error of the mean (SEM) unless indicated otherwise
  2. amyloid β, NS not significant