Fig. 2

Curvilinear three-way interaction of functional connectivity, amyloid-beta and time and its relationship with memory performance. To visualize the three-way interaction, the whole sample, excluding the AD group, was split based on whether functional connectivity between DMN-DAN was below or above zero, allowing us to visualize the dose-response relationship of Aβ burden (measured with ¹⁸F-AV-45 florbetapir standardized uptake value ratio) on memory decline in both subgroups. ¹⁸F-AV-45 florbetapir standardized uptake value ratio values over 1.11 indicate Aβ positivity. Linear or curvilinear graphs were drawn based on which type of association showed the best fit for each model. All p values are FDR-corrected. Significant effects are indicated by an asterisk and bold font. a Effects within the subgroup of cognitively normal participants and MCI patients showing positive correlations between networks. b Curvilinear effects within the subgroup of cognitively normal participants and MCI patients showing negative correlations between networks. c Effects within the cognitively normal subgroup with positive correlations between networks; no significant dose-response relationship of Aβ on memory (practice effects). d Effects within the MCI subgroup with positive correlations between networks showing a dose-response relationship of Aβ on memory. e Effects within the cognitively normal subgroup with negative correlations between networks showing a curvilinear dose-response relationship of Aβ on memory. f Effects within the MCI subgroup with negative correlations between networks showing no (borderline) significant effect of Aβ burden on memory