Fig. 1
From: Alzheimer disease pathology and the cerebrospinal fluid proteome
Study design and cerebrospinal fluid (CSF) proteome profiling workflow. CSF samples from 120 older individuals with or without cognitive impairment were analyzed using a highly automated shotgun MS-based proteomic workflow. The workflow consists of first removing 14 highly abundant proteins in CSF by immunoaffinity. The rest of the workflow is automated in a 96-well plate format and includes steps of (1) reduction, alkylation, and enzymatic digestion; (2) isobaric labeling and pooling; and (3) purifications. The samples are analyzed with reversed-phase LC-MS/MS, and the data are processed with standard bioinformatic tools