Fig. 6
S14 treatment modulates amyloid β (Aβ)-induced effects on autophagic markers. a Representative Western blot and b estimated p62 and light chain 3 (LC3)-II protein levels in SH-SY5Y cells. Protein level analysis revealed a significant increase in p62 and LC3-II levels in SH-SY5Y cells exposed to 1 μM oligomeric Aβ42. When cells were co-treated with 15 μM S14, p62 and LC3-II levels were recovered. Data are expressed as mean ± SEM; n = 6; **p < 0.01; ***p < 0.001; ****p < 0.0001. c Representative Western blot and d estimated p62 and LC3-II protein levels in the hippocampus of APP/PS1 and wild-type (wt) mice. p62 and LC3-II protein levels were found increased in the hippocampus of APP/PS1 mice and S14 treatment was able to restore both to those observed in wild-type mice. Data are expressed as mean ± SEM; n = 3–7 mice; *p < 0.05; ***p < 0.001. Statistical significance was assessed by two-way ANOVA followed by Fisher’s post-hoc test for multiple comparisons. Veh vehicle