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Fig. 6 | Alzheimer's Research & Therapy

Fig. 6

From: Anti-β-sheet conformation monoclonal antibody reduces tau and Aβ oligomer pathology in an Alzheimer’s disease model

Fig. 6

Immunoblots detecting oligomers on soluble supernatants from 20% brain homogenates of 19-month-old triple-transgenic (3 × Tg) mice infused with purified GW-23B7 or vehicle alone. a SDS-PAGE blotting of individual soluble supernatants from 20% brain homogenates of control infused 19-month-old 3 × Tg mice (lanes 1–8) and 19-month-old 3 × Tg mice infused with GW-23B7 (lanes 9–16). Top left panel: Fast Green protein reversible stain for comparable loading. Top right panel: Immunoblot with 4G8/6E10 antibodies specific for Aβ peptides. Bottom left panel: Immunoblot with PHF-1 antibody specific for hyperphosphorylated tau. Bottom right panel: Immunoblot with GW-23B7. Oligomeric forms analyzed were of different molecular weights and are identified by color-coded arrows; same color = same molecular weight. b Densitometric quantitation of the oligomer bands coded with colored arrows in (a). Statistical analysis was done using two-tailed, unpaired t tests. * p < 0.05; ** p < 0.01

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Alzheimer's Research & Therapy

ISSN: 1758-9193