Fig. 3
Amyloid-β (Aβ) content in the brain of APPSWE/PS1ΔE9 (APP/PS1) mice chronically treated with escitalopram. a–f Aβ plaques formed in the neocortex (a–d) and hippocampus (e and f) of both vehicle-treated (a, c, e) and escitalopram-treated (b, d, f) APP/PS1 mice, shown by 6E10 IHC. Cortical layers are indicated by roman numerals. DG Dentate gyrus, g Granule cell layer, h Hilus, molecular layer. Scale bars = 50 μm (a, b, e, f), 100 μm (c, d). g and h Levels of insoluble (guanidine fraction) and soluble (PBS fraction) Aβ40 and Aβ42 in the neocortex (g, i) and hippocampus (h, j) of APP/PS1 after escitalopram treatment were measured by Meso Scale Discovery multiplex analysis. Data are presented as mean ± SEM and were analysed by unpaired, two-tailed Mann-Whitney U test. *p < 0.05