Fig. 8
EBY100-L2 (AOE1) immunization does not activate β-amyloid peptide (Aβ)-reactive T cells and rarely induces microhemorrhages. T-cell activation in Alzheimer’s disease (AD) mice vaccinated with AOE1 was detected by enzyme-linked immunospot (ELISPOT) assay. The spleen cells were obtained from the AD mice vaccinated with AOE1 and restimulated with yeast carrier, Aβ42, AOE1 epitope peptides, and phorbol 12-myristate 13-acetate (PMA)/ionomycin (positive stimulator) as well as with dimethyl sulfoxide (DMSO) and PBS (negative control). The secretion of interferon (IFN)-γ (a) and interleukin (IL)-4 (b) were determined by ELISPOT assay. c, Microhemorrhages in the brain slices were determined through Prussian blue staining. Rare microhemorrhages were observed in the brains of mice vaccinated with AOE1. Arrows indicate microhemorrhage spots. d The synaptic pathology in the cortex and hippocampus of brain slices was determined through immunohistochemistry using antisynaptophysin antibody. WT Wild type