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Table 1 Primary antibodies used in this study

From: Tau passive immunization inhibits not only tau but also Aβ pathology

Antibody Type Specificity Phosphorylation sites Source/reference
43Da Monoclonal Tau   Covance (Princeton, NJ, USA)
77E9a Monoclonal Tau   Covance
R134d Polyclonal Tau   [77]
GAPDH Polyclonal GAPDH   Santa Cruz Biotechnology (Dallas, TX, USA)
pS199 Polyclonal p-tau Ser199 Invitrogen (Carlsbad, CA, USA)
AT8 Monoclonal p-tau Ser202/Thr205 Thermo Fisher Scientific (Waltham, MA, USA)
pT205 Polyclonal p-tau Thr205 Invitrogen
12E8 Monoclonal p-tau Ser262/356 Dr. D. Schenk, Elan Pharmaceuticals (South San Francisco, CA, USA)
PHF1 Monoclonal p-tau Ser396/404 Dr. P. Davies, Albert Einstein College of Medicine (Bronx, NY, USA)
APP Polyclonal APP   Generated in our institute
4G8 Monoclonal APP/Aβ   BioLegend (San Diego, CA, USA)
Iba1 Polyclonal Iba1   Abcam (Cambridge, MA, USA)
Iba1 Polyclonal Iba1 for IHC   Wako Chemicals (Richmond, VA, USA)
C1q Monoclonal C1q   Thermo Fisher Scientific
C9 Polyclonal C9   Thermo Fisher Scientific
  1. Abbreviations: APP Amyloid precursor protein, Amyloid-β, GAPDH Glyceraldehyde 3-phosphate dehydrogenase, IHC Immunohistochemistry, PHF1 Paired helical filament 1, pS199 Phospho-tau (Ser199), pT205 Phospho-tau (Thr205), p-tau Phosphorylated tau
  2. aImmunoglobulin G1 kappa light chain