Fig. 6
From: Tau passive immunization inhibits not only tau but also Aβ pathology
Immunization with 43D increases the activity of microglia and the complement system in 3×Tg-AD mice. The activation of microglia and the complement system was assessed 1 day after the sixth injection. a Thioflavin S staining and Iba1 immunofluorescence were performed in the subiculum area. White arrows highlight activated microglial cells aggregated around Aβ plaques. Representative Western blots of hippocampus developed (b) with Iba1 antibody and (c) with complement components C1q and C9 antibodies and densitometric quantification of blots after normalization with GAPDH. Three or five representative 3×Tg-AD mice in the IgG or 43D treatment group, respectively, are shown. Data are percentages of mouse IgG (100%)-treated animals, reported as mean ± SEM. *p < 0.05 by unpaired two-tailed Student’s t test. AD Alzheimer’s disease, Aβ Amyloid-β, GAPDH Glyceraldehyde 3-phosphate dehydrogenase, IgG Immunoglobulin G, 3×Tg Triple-transgenic