Fig. 4
From: Tau passive immunization inhibits not only tau but also Aβ pathology
Immunization with 43D dose-dependently decreases tau pathology. Immunization with (a) two doses of 43D and 77E9 antibody or (b–e) six doses of 43D. Mice were killed 1 day after their last injection. a and b Representative Western blots of hippocampus developed with R134d and 92e against total tau, 43D against human transgenic tau, and several phosphorylation-dependent and site-specific tau antibodies. Densitometric quantification of blots after normalization with GAPDH is shown. Five of the 3×Tg-AD mice per group in (a) and three or five of the 3×Tg-AD mice for IgG or 43D treatment, respectively, are shown. Data are percentages of mouse IgG (100%)-treated animals reported as mean ± SEM. Immunostaining of CA1 from 3×Tg-AD mice immunized with six doses of 43D or control IgG with (c) R134d, (d) 43D, or (e) PHF1 antibodies. The quantification of R134d, 43D, and PHF1 immunostaining intensity in total CA1 region was based on two sections from each of three or five mice per group. Data are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 by ANOVA followed by a Bonferroni post hoc test for two doses of immunization or by unpaired two-tailed t test for six doses of immunization. AD Alzheimer’s disease, ANOVA Analysis of variance, GAPDH Glyceraldehyde 3-phosphate dehydrogenase, IgG Immunoglobulin G, 3×Tg Triple-transgenic