Fig. 1
From: CRTC1 gene is differentially methylated in the human hippocampus in Alzheimer’s disease
DNA methylation levels within two promoter regions of CRTC1 in Alzheimer’s disease (AD) and control hippocampus. a The graph shows genomic position of the two amplicons (Prom1 and Prom2) within the two promoter regions of the CRTC1 gene that were examined by bisulfite cloning sequencing. At the bottom of the graph, predicted functional elements are shown for each of nine human cell lines explored by chromatin immunoprecipitation (ChIP) combined with massively parallel DNA sequencing. Boxes represent promoter regions (red), enhancers (yellow), transcriptional transition and elongation (dark green) and weak transcribed regions (light green). CpG islands are also represented by green boxes. The track was obtained from Chromatin State Segmentation by HMM from ENCODE/Broad track shown at the UCSC Genome Browser. Next, dot plot charts and representative examples of the methylation graphs for Prom1 (b) and Prom2 (c), respectively, are shown. A decrease in methylation was observed within both promoters in AD hippocampus compared to controls. Horizontal lines within dot plots represent median methylation values for each group. Boxes on the right represent individual patients. Black and white circles denote methylated and unmethylated cytosines, respectively. Each column symbolizes a unique CpG site in the examined amplicon and each line represents an individual DNA clone. *p-value < 0.05; ** p-value < 0.005. CpG cytosine-phosphate-guanine