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Table 2 Brain tissue blocks and stains used for neuropathology

From: Assessing clinicopathological correlation in chronic traumatic encephalopathy: rationale and methods for the UNITE study

  Stain  
Reserve Luxol fast blue Bielschowsky silver stain AT8 Amyloid-β (4G8)a α-Synucleina pTDP-43a Minimum stainingb
Brain region Paraffin blocks, n
Olfactory bulb 1   X   X   X   X
Midbrain at level of red nucleus 1   X   X X X X  
Midbrain at superior cerebellar peduncle 1 X        
Precentral, postcentral cortices (BA 4, 3, 2, 1) 1   X   X     
Inferior parietal cortex (BA 39, 40) 1   X X X X    X
Anterior cingulate (BA 24) 1   X     X   
Superior frontal (BA 8, 9) 1   X   X     X
Inferior frontal cortex (BA 10, 11, 12) 2   X   X     X
Dorsolateral frontal (BA 45, 46) 2   X X X X   X X
Caudate-putamen-accumbens, septal cortex 2   X   X X    
Insular cortex 2   X   X     
Temporal pole (BA 38) 1   X   X     X
Superior temporal (BA 20, 21, 22) 1   X   X X    X
Amygdala, with entorhinal cortex (BA 28) 1   X   X X X X X
Globus pallidus, insula, substantia innominata 1   X   X     
Anterior hippocampus 1 X        
Hippocampal formation, lateral geniculate 1   X X X X   X X
Superior temporal posterior (BA 41, 42) 1 X        
Thalamus with subthalamic nucleus 1 X        
Hypothalamus, mammillary body 1   X   X     
Posterior thalamus 1 X        
Posterior cingulate (BA 23, 31) 1 X        
Calcarine cortex (BA 17, 18) 1   X X X     
Superior parietal cortex (BA 7B) 1 X        
Upper pons (level of locus coeruleus) 1   X   X     X
Pons, middle cerebellar peduncle 1 X        
Medulla oblongata with inferior olives 1   X   X   X   X
Cervical spinal cord 1   X   X    X  
Thoracic spinal cord 1 X        
Lumbar spinal cord 1   X       
Sacral spinal cord 1 X        
Cerebellar vermis 1   X   X     
Cerebellum with dentate nucleus 1   X X X X    X
Parastriate cortex 1 X        
  1. Abbreviations: BA Brodmann area, pTDP-43 phosphorylated transactive response DNA binding protein 43 kDa
  2. aIf screening regions are positive for β-amyloid, α-synuclein, or pTDP-43, additional regions are stained to allow for complete staging of these pathologies
  3. bMinimum regions that must be evaluated to make a neuropathological chronic traumatic encephalopathy diagnosis