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Table 2 Summary of key studies on electrophysiological profiles of human iPSC-derived neurons

From: Connectivity and circuitry in a dish versus in a brain

  Pre and colleagues, 2014 [9] Tang and colleagues, 2013 [10] Zhang and colleagues, 2013 [120]
Method Human iPSC neuronal differentiation via smad inhibition Human iPSC-derived NPC neuronal differentiation Human iPSC-iN cells (via Neurogenin-2 expression)
Cell line 7889O WT126, WT33 Not stated
DIV 31 to 38 41 to 45 55 55 42 60 42 60 21
AP firing (% of cells) 20 48 60 86 NQ NQ NQ NQ >95
Single (%) NQ NQ NQ NQ NQ NQ NQ NQ NQ
Repetitive (%) NQ NQ NQ NQ 16   73   NQ
Spontaneous AP (%) NQ NQ NQ NQ NQ NQ NQ NQ NQ
n 18 27 20 15 25 NQ 22 NQ 33 to 34
Rin (MΩ) 2,500.0 2,200.0 1,700.0 NQ NQ 695.0 NQ 302.0 NQ
Cm (pF) NQ NQ NQ NQ NQ 27.0 NQ 119.0 NQ
τ (milliseconds) 34.0 29.0 21.0 NQ NQ NQ NQ NQ NQ
RMP (mV) −35.0 −38.0 −49.0 NQ NQ −44.0 NQ −59.0 NQ
n 89 75 103 NQ NQ 22 NQ 23 NQ
Spontaneous activity (%) 11 16 21 27 NQ NQ NQ NQ >95
Evoked potential (%) NQ NQ NQ NQ NQ NQ NQ NQ >95
n 8 6 20 15 NQ NQ NQ NQ 33 to 34
Co-culture x Mouse glial cells x x Mouse astrocytes Mouse astrocytes Mouse glial cells   
  1. AP, action potential; Cm, membrane capacitance; DIV, days in vitro; iN, induced neuronal; iPSC, induced pluripotent stem cell; NPC, neural progenitor cell; NQ, not quantified; Rin, input resistance; RMP, resting membrane potential; τ, membrane time constant; x, not measured, used or stated