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Table 1 Human CSF proteins displaying differential expression in AD patients

From: Differentially charged isoforms of apolipoprotein E from human blood are potential biomarkers of Alzheimer’s disease

Spot number Protein name/Protein ID/Uniprot Accession Number/RefSeq 33 N versus 33D 33 N versus 44D N versus D Molecular weight pI
429 Serum albumin, Chain A; ALBU_HUMAN P02768/NP_000468.1 -1.29 -1.37 -1.33 69,366.68 5.92
P = 0.00034
541 Transferrin variant; Q53H26_HUMAN Q53H26 /IP|00022463 -1.53 -1.50 -1.51 77,079.85 6.68
P = 0.0011
575 alpha 1-B glycoprotein; A1BG_HUMAN P04217/NP_570602.2 -1.22 -1.18 -1.20 54,272.56 5.58
P = 0.0013
579 alpha 1-B glycoprotein; A1BG_HUMAN P04217/NP_570602.2 -1.31 -1.31 -1.31 54,272.56 5.56
P = 0.0015
614 Transferrin variant; Q53H26_HUMAN Q53H26/IP|00022463 +1.70 +1.64 +1.67 770,79.85 7.04
P = 0.0024
990 Apolipoprotein H (beta-2-glycoprotein I) APOH_HUMAN P02749/NP_000033.2 +1.67 +1.60 +1.63 38,298.16 8.34
P = 0.0021
1000 Tubulin beta-2A chain; TBB2A_HUMAN Q13885/NP_001060.1 +1.29 +1.73 +1.51 49,906.67 4.78
P = 0.0058
1008 Keratin, type II cytoskeletal 1; K2C1_HUMAN P04264/NP_006112.3 +2.94 +2.62 +2.77 66,038.73 8.15
P = 0.002
1017 alpha-1-antitrypsin (SerpinA1); A1AT_HUMAN P01009/NP_000286.3 +1.86 +2.26 +2.06
P = 0.0021
46,739.55 5.37
1118 Pigment epithelium-derived factor (SerpinF1); PEDF_HUMAN P36955/NP_002606.3 +1.54 +1.99 +1.77 46,342.30 5.97
P = 7.4E-05
1188 ALB protein (growth-inhibiting protein 20), Isoform 2; ALBU_HUMAN P02768/NP_000468.1 +1.26 +1.23 +1.24 47,360.49 5.97
P = 0.0065
1268 Glutamine synthetase; GLNA_HUMAN; P15104/NP_001028216.1 -1.38 -1.55 -1.46 42,064.46 6.43
P = 0.0025
1278 Creatine kinase B-type; KCRB_HUMAN; P12277/NP_001814.2 -1.46 -1.40 -1.43 42,644.28 5.34
P = 0.0077
1324 Fructose-bisphosphate aldolase A; ALDOA_HUMAN P04075/NP_000025.1 -1.49 -1.53 -1.51 39,420.02 8.30
P = 0.0074
1332 Aspartate aminotransferase, cytoplasmic; AATC_HUMAN; P17174/NP_002070.1 -1.28 -1.49 -1.38 46,247.51 6.53
P = 0.0099
1363 Aspartate aminotransferase, cytoplasmic; AATC_HUMAN; P17174/NP_002070.1 -1.27 -1.41 -1.33 46,247.51 6.50
P = 0.0003
1451 Glyceraldehyde-3-phosphate dehydrogenase G3P_HUMAN P04406/NP_002037.2 -2.05 -1.75 -1.87 36,042.22 8.57
P = 0.0002
1454 Glyceraldehyde-3-phosphate dehydrogenase G3P_HUMAN P04406/NP_002037.2 -1.45 -1.42 -1.44 36,042.22 8.59
P = 0.013
1469 Glyceraldehyde-3-phosphate dehydrogenase G3P_HUMAN P04406/NP_002037.2 -1.58 -1.52 -1.55 36,042.22 8.63
P = 0.00094
1470 Glyceraldehyde-3-phosphate dehydrogenase G3P_HUMAN P04406/NP_002037.2 -1.80 -1.72 -1.75 36,042.22 8.67
P = 0.0005
1472 Clusterin; Apolipoprotein J; Complement-associated protein SP-40; CLUS_HUMAN; P10909/NP_001164609.1 +2.01 +2.01 +2.01 50,062.56 5.89
P = 5.10E-05
1521 Apolipoprotein E4; APOE_HUMAN P02649/NP_000032.1 +2.14 +3.34 +2.74 36,154.08 5.65
P = 0.00023
1523 Clusterin; Apolipoprotein J; Complement associated protein SP-40; CLUS_HUMAN; P10909/NP_001164609.1 +1.78 +2.17 +1.97 50,062.56 5.89
P = 3.50E-06
1527 Apolipoprotein E4; APOE_HUMAN P02649/NP_000032.1 +2.02 +2.42 +2.22 36,154.08 5.65
P = 1.30E-05
1535 Complement component 4A; C4A; Q5JNX2_HUMAN; Q5JNX2/IP|00643525 +1.85 +1.94 +1.89 ~3,5000 6.43
P = 7.10E-05
1554 Transthyretin; TTHY_HUMAN; P02766/NP_000362.1 +2.05 +2.58 +2.32 15,887.03 5.52
P = 0.00078
1779 Ig Kappa chain C region; IGKC; IGKC_HUMAN; P01834/IP|00909649 -1.98 -2.14 -2.06 11,608.86 5.58
P = 0.0061
1845 Prostaglandin-H2 D-isomerase; cerebrin-28; PTGDS_HUMAN; P41222/NP_000945.3 +1.45 +1.51 +1.48 21,028.82 7.66
P = 0.0077
1912 Apolipoprotein A-1; ApoA1; APOA1-HUMAN; P02647/NP_000030.1 -1.78 -1.84 -1.81 30,777.83 5.56
P = 0.0037
1982 Superoxide dismutase [Mn], mitochondrial; SODM_HUMAN; P04179/NP_000627.2 +1.66 +1.62 +1.64 24,722.09 8.35
P = 0.0011
2067 Apolipoprotein A-1; ApoA1; APOA1-HUMAN; P02647/NP_000030.1 -1.95 -2.09 -2.02 30,777.83 5.56
P = 7.40E-06
2068 Apolipoprotein A-1; ApoA1; APOA1-HUMAN; P02647/NP_000030.1 -2.25 -2.24 -2.24 30,777.83 5.56
P = 0.00035
  1. The spot number is assigned by default by the DeCyder 2D 7.0 software, and is indicated on the gel image (Figure 3B). The protein name and the protein ID are obtained from the protein database maintained at the Protein Information Resource server, Georgetown University [37].
  2. The Uniprot accession number and the RefSeq number are protein identifiers retrieved from the Uniprot database based on the protein identification achieved by using mass spectrometry data [38]. Changes in protein expression are expressed as the log of the ratios between the corresponding spots’ volumes, and are automatically calculated with DeCyder 2D.
  3. The protein’s isoelectric point (pI) and molecular weight (Mw) are associated with the protein ID in PIR [37].