Figure 3

Increased freezing in tubastatin-treated rTg4510 mice during the acquisition phase of fear conditioning. (A) Treatment with tubastatin significantly increases the percent freezing ability of the rTg4510 mice compared with the saline-treated littermates (n = 6/group) in the acquisition phase of the fear conditioning training paradigm. Two-way analysis of variance followed by Fisher’s PLSD test revealed a significant treatment effect in both transgenic (tg; P < 0.0001) and nontransgenic (ntg; P < 0.038) animal groups compared with saline-treated mice of the same genotype. (B) All mice exhibited high levels of freezing during the retention phase of the test, indicating recall of the context. *P < 0.05, ***P < 0.001.